Comparative proteomic analysis reveals that juvenile hormone binding protein and adenylate kinase may be involved in the molting process of silkworm, Bombyx mori.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2017-10-13 DOI:10.25431/1824-307X/ISJ.V14I1.388-403

Yanhua Yang, Liang Chen, Qi Tang, Y. Zhang, Hanhan Tang, P. Lü, Q. Yao, Kangmin Chen

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引用次数: 3

Abstract

The molting is an essential part of the silkworm metamorphosis development. Although previous studies have demonstrated that molting in silkworm is associated with prothoracicotropic hormone (PTTH), molting hormone (MH), and juvenile hormone (JH), the changes of proteins and genes during silkworm molting, as well as the molecular mechanism about its generating and maintaining remains unclear. In this paper, the proteomic approaches were employed to investigate this issue. Totally, 35 different proteins were successfully identified through mass spectrometry and database searches, among which 42 % proteins were involved in cell structure and 16 % proteins belonged to the metabolism group. Meanwhile, vacuolar ATP synthase, juvenile hormone binding protein precursor and adenylate kinase isoenzyme were found to be down-regulated at early, mid-molt stages, which were further confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). Taken together, our data suggests that juvenile hormone binding protein (JHBP) and adenylate kinase (AK) play a critical role in the process of silkworm molting, which may participate in the regulation of silkworm molting.

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比较蛋白质组学分析表明，幼体激素结合蛋白和腺苷酸激酶可能参与了家蚕的蜕皮过程。

蜕皮是家蚕变态发育的重要组成部分。虽然已有研究表明家蚕的蜕皮与促胸性激素(pth)、蜕皮激素(MH)和幼崽激素(JH)有关，但目前尚不清楚家蚕蜕皮过程中蛋白质和基因的变化及其产生和维持的分子机制。本文采用蛋白质组学方法对这一问题进行了研究。通过质谱分析和数据库检索，共鉴定出35种不同的蛋白，其中42%的蛋白与细胞结构有关，16%的蛋白属于代谢组。同时，液泡ATP合成酶、幼体激素结合蛋白前体和腺苷酸激酶同工酶在蜕皮早中期下调，定量实时聚合酶链反应(qRT-PCR)进一步证实了这一点。综上所述，我们的数据表明，幼体激素结合蛋白(JHBP)和腺苷酸激酶(AK)在家蚕的蜕皮过程中起着关键作用，可能参与了家蚕蜕皮的调控。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.