Development of Spectrophotometric Method to Assay Sulfadiazine in Pure and in Pharmaceutical Dosage form through Diazotization and Coupling Reaction

Dheyaa Azeez, Salim J. Mohammed
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引用次数: 1

Abstract

This study involves the development of a sensitive accurate and low-cost spectrophotometric procedure to analyze sulfadiazine as pure and in its dosage forms. In this method sulfadiazine was diazotized with an equimolar solution of sodium nitrite in the presence of hydrochloric acid to form corresponding diazonium salt followed by coupling with phloroglucinol reagent in basic solution of NaOH to produce a yellow water-soluble azo dye that has maximum absorption at 417 nm versus reagent blank. Under optimum conditions. The linearity of the method obeyed Beer’s law in the concentration range 0.25-15 μg/ml with an excellent determination coefficient (R 2 = 0.9992) and molar absorptivity 3.81×10 4 l/mol.cm. The detection limit (LOD) and quantification limit (LOQ) have also been estimated and their values were found to be 0.192 and 0.563 μg/ml, respectively. A relative error% was calculated and found in the range -2.75% to 4.02%, while the precision (RSD%) was estimated as ≤ 1.36%. The stoichiometry of the resulting azo dye was found to be 1:1 sulfadiazine: with stability constant 0.37×10 7 l/mol. The suggested procedure was applied to the analysis of sulfadiazine in cream and veterinary drugs (powder and injection).
通过重氮化和偶联反应测定纯磺胺嘧啶和药物剂型的分光光度法的建立
本研究涉及开发一种灵敏、准确和低成本的分光光度法来分析纯磺胺嘧啶及其剂型。该方法首先在盐酸存在下,用亚硝酸钠等摩尔溶液将磺胺嘧啶重氮化,形成相应的重氮盐,然后与间苯三酚试剂在碱性NaOH溶液中偶联,制得黄色水溶性偶氮染料,该染料在417 nm处比空白试剂有最大的吸收。在最佳条件下。该方法在0.25 ~ 15 μg/ml范围内线性符合比尔定律,具有良好的测定系数(r2 = 0.9992)和摩尔吸光度3.81×10 4 l/mol.cm。测定了其检出限(LOD)和定量限(LOQ),分别为0.192和0.563 μg/ml。相对误差%在-2.75% ~ 4.02%范围内,精密度(RSD%)≤1.36%。偶氮染料的化学计量为1:1磺胺嘧啶:稳定常数0.37×10 7 l/mol。本方法适用于乳膏和兽药(粉剂和注射剂)中磺胺嘧啶的分析。
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