Concordancia entre la prueba del anillo en leche y ELISA indirecto en el diagnóstico de brucelosis bovina

Q2 Veterinary

Revue De Medecine Veterinaire Pub Date : 2016-11-30 DOI:10.19052/MV.4057

Rubén Darío Moreira Zúñiga

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引用次数: 1

Abstract

Concordance analysis are used to compare diagnostic methods when applied to the same group of individuals (paired samples). This article analyses the case of two laboratory methods, whose results are expressed in form of dichotomous qualitative variable (positive/ negative). In order to analyze the problem of concordance, the dual-vision method for analysis of agreement was used, which is based on a main assumption: two diagnostic methods agree when they have the same sensibility and specificity. The study analyzed 153 milk samples corresponding to the same number of herds in the central area of Chile; these were classified into three strata, defined by the number of lactating cows at the time of obtaining the sample. Standard methodology of milk ring test (MRT) and indirect ELISA (ELISA-i) were performed. To determine the concordance between these two diagnostic methods, values observed in all three strata (A, B, and C) were used; for each one, concordance rate (l) was calculated. Concordance rates were high in all strata, so the study concludes that both MRT and ELISA-i could be used to diagnose bovine brucellosis.

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乳环试验与间接ELISA在牛布鲁氏菌病诊断中的相关性

一致性分析用于比较同一组个体(配对样本)的诊断方法。本文分析了两种实验室方法的案例，其结果以二分定性变量(阳性/阴性)的形式表示。为了分析一致性问题，采用双视觉一致性分析方法，该方法基于一个主要假设:两种诊断方法在具有相同的敏感性和特异性时是一致的。该研究分析了智利中部地区相同数量畜群的153个牛奶样本;这些被分为三个层次，由获得样品时泌乳奶牛的数量来定义。采用乳环试验(MRT)和间接酶联免疫吸附试验(ELISA-i)的标准方法。为了确定这两种诊断方法之间的一致性，使用了在所有三个地层(A, B和C)中观察到的值;计算每一项的一致性率(l)。结果表明，MRT和ELISA-i均可用于牛布鲁氏菌病的诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revue De Medecine Veterinaire 农林科学-兽医学

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

18-36 weeks

期刊介绍： The Revue de Médecine Vétérinaire publishes four kinds of text: 1) Scientific reviews on subjects related to veterinary and comparative medicine. Suggested length: 10 to 30 typed pages. 2) Original reports on fundamental or applied research. Suggested length: 10 to 15 typed pages. 3) Continuous education articles, that should be easily understandable by non-specialists. Suggested length: 10 to 15 typed pages. 4) Clinical reports. Suggested length: 5 to 15 typed pages. The publication can be done in French language or English language. For an article written in English by not english native speakers authors, the manuscript must be subjected by attesting that it was read again by an anglophone scientist or a scientific translator. The authors must certify that the manuscript was not published or subjected for publication to another review. The manuscript must be accompanied by a sheet signed by all the joint authors indicating their agreement for the tender of the manuscript. The publication is free but a financial participation could be required for the photographs color. An estimate will be sent to collect the agreement of the authors.