Compositional and toxicological investigation of pooled venom from farm-raised Naja atra

IF 1.8 3区医学 Q4 TOXICOLOGY

Journal of Venomous Animals and Toxins Including Tropical Diseases Pub Date : 2022-03-14 DOI:10.1590/1678-9199-JVATITD-2021-0040

Gang Xiao, Junqi Liu, Lingfeng Peng, Yang Yang, Zhiliang Sun

{"title":"Compositional and toxicological investigation of pooled venom from farm-raised Naja atra","authors":"Gang Xiao, Junqi Liu, Lingfeng Peng, Yang Yang, Zhiliang Sun","doi":"10.1590/1678-9199-JVATITD-2021-0040","DOIUrl":null,"url":null,"abstract":"Abstract Background: Naja atra is a venomous snake species medically relevant in China. In the current study, we evaluated the composition and toxicological profile of venom collected from farm-raised N. atra. Methods: Venom was collected from third-generation captive bred N. atra on a snake farm in Hunan Province, China. The venom was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis and nano-liquid chromatography with electrospray ionization tandem mass spectrometry. In addition, hemolytic activity, median lethal dose, serum biochemical and histopathological parameters were accessed. Results: N. atra venom proteome was dominated by phospholipase A2 (46.5%) and three-finger toxins (41.4 %), and a set of common low relative abundance proteins, including cysteine-rich secretory proteins (4.7%), NGF-beta (2.4%), snake venom metalloproteinase (1.5%), glutathione peroxidase (0.6%), vespryn (0.3%), and 5ʹ-nucleotidases (0.2%) were also found. Furthermore, the venom exhibited direct hemolytic activity, neurotoxicity, myotoxicity, and high lethal potency in mice, with a subcutaneous median lethal dose of 1.02 mg/kg. Histopathological analysis and serum biochemical tests revealed that venom caused acute hepatic, pulmonary and renal injury in mice. Conclusion: This study revealed the composition and toxicity of venom collected from farm-raised N. atra, thereby providing a reference for the analysis of venom samples collected from captive-born venomous snakes in the future.","PeriodicalId":17565,"journal":{"name":"Journal of Venomous Animals and Toxins Including Tropical Diseases","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Venomous Animals and Toxins Including Tropical Diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1590/1678-9199-JVATITD-2021-0040","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"TOXICOLOGY","Score":null,"Total":0}

引用次数: 2

Abstract

Abstract Background: Naja atra is a venomous snake species medically relevant in China. In the current study, we evaluated the composition and toxicological profile of venom collected from farm-raised N. atra. Methods: Venom was collected from third-generation captive bred N. atra on a snake farm in Hunan Province, China. The venom was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis and nano-liquid chromatography with electrospray ionization tandem mass spectrometry. In addition, hemolytic activity, median lethal dose, serum biochemical and histopathological parameters were accessed. Results: N. atra venom proteome was dominated by phospholipase A2 (46.5%) and three-finger toxins (41.4 %), and a set of common low relative abundance proteins, including cysteine-rich secretory proteins (4.7%), NGF-beta (2.4%), snake venom metalloproteinase (1.5%), glutathione peroxidase (0.6%), vespryn (0.3%), and 5ʹ-nucleotidases (0.2%) were also found. Furthermore, the venom exhibited direct hemolytic activity, neurotoxicity, myotoxicity, and high lethal potency in mice, with a subcutaneous median lethal dose of 1.02 mg/kg. Histopathological analysis and serum biochemical tests revealed that venom caused acute hepatic, pulmonary and renal injury in mice. Conclusion: This study revealed the composition and toxicity of venom collected from farm-raised N. atra, thereby providing a reference for the analysis of venom samples collected from captive-born venomous snakes in the future.

查看原文本刊更多论文

农养大白鲨毒液的成分及毒理学研究

背景:大毒蛇是中国一种具有医学意义的毒蛇。在目前的研究中，我们评估了从农场饲养的北甲收集的毒液的成分和毒理学特征。方法:采集湖南省某蛇场第三代圈养大腹蛇毒液。采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳和纳米液相色谱-电喷雾电离串联质谱法对毒液进行分析。测定溶血活性、中位致死剂量、血清生化及组织病理学指标。结果:北蛇毒液蛋白质组以磷脂酶A2(46.5%)和三指毒素(41.4%)为主，并发现了一组常见的低相对丰度蛋白，包括富半胱氨酸分泌蛋白(4.7%)、ngf - β(2.4%)、蛇毒金属蛋白酶(1.5%)、谷胱甘肽过氧化物酶(0.6%)、vespryn(0.3%)和5′-核苷酸酶(0.2%)。此外，该毒液对小鼠具有直接溶血活性、神经毒性、肌肉毒性和高致死力，皮下致死中位剂量为1.02 mg/kg。组织病理学分析和血清生化试验显示，毒液可引起小鼠急性肝、肺和肾损伤。结论:本研究揭示了圈养毒蛇毒液的组成和毒性，为今后圈养毒蛇毒液样品的分析提供参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Venomous Animals and Toxins Including Tropical Diseases 医学-毒理学

CiteScore

4.80

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Journal of Venomous Animals and Toxins including Tropical Diseases (JVATiTD) is a non-commercial academic open access publication dedicated to research on all aspects of toxinology, venomous animals and tropical diseases. Its interdisciplinary content includes original scientific articles covering research on toxins derived from animals, plants and microorganisms. Topics of interest include, but are not limited to:systematics and morphology of venomous animals;physiology, biochemistry, pharmacology and immunology of toxins;epidemiology, clinical aspects and treatment of envenoming by different animals, plants and microorganisms;development and evaluation of antivenoms and toxin-derivative products;epidemiology, clinical aspects and treatment of tropical diseases (caused by virus, bacteria, algae, fungi and parasites) including the neglected tropical diseases (NTDs) defined by the World Health Organization.