Genetic variance distribution of SSR markers and economically important quantitative traits in a progeny trial of Prosopis chilensis (Leguminosae): implications for the ‘Algarrobo’ management programme

IF 3 2区农林科学 Q1 FORESTRY

Forestry Pub Date : 2020-07-24 DOI:10.1093/forestry/cpaa026

Daniela Chequer Charan, C. Pometti, M. Cony, J. Vilardi, B. Saidman, C. Bessega

{"title":"Genetic variance distribution of SSR markers and economically important quantitative traits in a progeny trial of Prosopis chilensis (Leguminosae): implications for the ‘Algarrobo’ management programme","authors":"Daniela Chequer Charan, C. Pometti, M. Cony, J. Vilardi, B. Saidman, C. Bessega","doi":"10.1093/forestry/cpaa026","DOIUrl":null,"url":null,"abstract":"\n Prosopis chilensis (Molina) Stuntz (Leguminosae) is a valuable native species in Argentina included in the Prosopis Management Programme. Natural provenances show important height and shape differentiation throughout their distribution in the Monte Desert. The availability of progeny trials provides an opportunity to quantify genetic differentiation among provenances and test the relative importance of demographic vs adaptive processes on morphological variation. We quantified both genetic and quantitative differentiation of neutral markers and five economically important traits, respectively, among four provenances in a provenance-progeny trial. We aimed to quantify the genetic basis of variations in height, basal diameter, tree shape, spine length and biomass. Two hypotheses were tested: (1) economically important traits have significant heritability, and (2) the phenotypic variation among provenances is the result of local adaptation to particular environmental conditions. Our results indicate that most morphological variation was found among individuals within families (~95 per cent). The h2 estimates were heterogeneous among traits and ranged from low (0 for number of stems) to moderate (0.22 and 0.28 for spine length and biomass, respectively). Variance among families (~5 per cent) was evenly distributed within and among provenances. Morphological differentiation among provenances was low, but significant, and could be attributed mainly to individuals from Villa Unión. Based on molecular markers, genetic differentiation among provenances was low and significant (FST = 0.03; P = 5 × 10−4) but was able to differentiate the groups from Villa Unión, Fiambalá and Mogna-Chilecito. Neutrality tests were conducted using the FST –QST test and DJSOST and δGREGORIUS alternative coefficients of differentiation. Neutrality tests yielded no evidence of local adaptation and were rather consistent in showing a trend toward stabilizing selection, particularly for spine length. The selection strategy for breeding programmes should depend on the trait to be improved and should consider both provenance and familiar information. Considering an intra-familiar ranking is encouraged in order to maximize the genetic gain. Additionally, in order to recover germplasm provenance diversity, based on morphological and microsatellite results, our recommendation would be to include seeds from individuals from at least the Villa Unión, Fiambalá and Mogna-Chilecito areas.","PeriodicalId":12342,"journal":{"name":"Forestry","volume":"37 1","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2020-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Forestry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/forestry/cpaa026","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FORESTRY","Score":null,"Total":0}

引用次数: 2

Abstract

Prosopis chilensis (Molina) Stuntz (Leguminosae) is a valuable native species in Argentina included in the Prosopis Management Programme. Natural provenances show important height and shape differentiation throughout their distribution in the Monte Desert. The availability of progeny trials provides an opportunity to quantify genetic differentiation among provenances and test the relative importance of demographic vs adaptive processes on morphological variation. We quantified both genetic and quantitative differentiation of neutral markers and five economically important traits, respectively, among four provenances in a provenance-progeny trial. We aimed to quantify the genetic basis of variations in height, basal diameter, tree shape, spine length and biomass. Two hypotheses were tested: (1) economically important traits have significant heritability, and (2) the phenotypic variation among provenances is the result of local adaptation to particular environmental conditions. Our results indicate that most morphological variation was found among individuals within families (~95 per cent). The h2 estimates were heterogeneous among traits and ranged from low (0 for number of stems) to moderate (0.22 and 0.28 for spine length and biomass, respectively). Variance among families (~5 per cent) was evenly distributed within and among provenances. Morphological differentiation among provenances was low, but significant, and could be attributed mainly to individuals from Villa Unión. Based on molecular markers, genetic differentiation among provenances was low and significant (FST = 0.03; P = 5 × 10−4) but was able to differentiate the groups from Villa Unión, Fiambalá and Mogna-Chilecito. Neutrality tests were conducted using the FST –QST test and DJSOST and δGREGORIUS alternative coefficients of differentiation. Neutrality tests yielded no evidence of local adaptation and were rather consistent in showing a trend toward stabilizing selection, particularly for spine length. The selection strategy for breeding programmes should depend on the trait to be improved and should consider both provenance and familiar information. Considering an intra-familiar ranking is encouraged in order to maximize the genetic gain. Additionally, in order to recover germplasm provenance diversity, based on morphological and microsatellite results, our recommendation would be to include seeds from individuals from at least the Villa Unión, Fiambalá and Mogna-Chilecito areas.

查看原文本刊更多论文

紫豆(豆科)子代试验中SSR标记和重要经济数量性状的遗传变异分布:对“Algarrobo”管理计划的启示

豆科毛豆(Prosopis chilensis (Molina) Stuntz)是阿根廷的一种珍贵的本地物种，已被列入豆科毛豆管理计划。自然物源在蒙地沙漠的分布中表现出重要的高度和形状分异。后代试验的可用性提供了一个机会来量化种源之间的遗传分化，并测试人口统计学与适应过程对形态变异的相对重要性。在种源-后代试验中，我们分别量化了4个种源间中性标记和5个重要经济性状的遗传和数量分化。我们的目的是量化在高度、基径、树形、脊柱长度和生物量变异的遗传基础。检验了两个假设:(1)经济上重要的性状具有显著的遗传力;(2)种源间的表型变异是对特定环境条件的局部适应的结果。结果表明，大多数形态变异发生在科内个体之间(约95%)。不同性状间的h2值存在异质性，从低(茎数为0)到中等(棘长和生物量分别为0.22和0.28)。家系间变异(~ 5%)在种源内和种源间均匀分布。种源间形态差异虽小，但很显著，主要是维拉Unión的个体。在分子标记上，种源间的遗传分化较低且显著(FST = 0.03;P = 5 × 10−4)，但能够区分Villa Unión、fiambal和Mogna-Chilecito。采用FST -QST检验、DJSOST和δGREGORIUS交替分化系数进行中性检验。中性试验没有发现局部适应的证据，而且相当一致地显示了稳定选择的趋势，特别是在脊柱长度方面。育种计划的选择策略应取决于需要改进的性状，并应考虑种源和熟悉信息。考虑到一个内部熟悉的排名是鼓励，以最大限度地提高遗传收益。此外，为了恢复种质来源多样性，基于形态学和微卫星结果，我们建议至少包括来自Villa Unión, fiambal和Mogna-Chilecito地区的个体种子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Forestry 农林科学-林学

CiteScore

6.70

自引率

7.10%

发文量

审稿时长

12-24 weeks

期刊介绍： The journal is inclusive of all subjects, geographical zones and study locations, including trees in urban environments, plantations and natural forests. We welcome papers that consider economic, environmental and social factors and, in particular, studies that take an integrated approach to sustainable management. In considering suitability for publication, attention is given to the originality of contributions and their likely impact on policy and practice, as well as their contribution to the development of knowledge. Special Issues - each year one edition of Forestry will be a Special Issue and will focus on one subject in detail; this will usually be by publication of the proceedings of an international meeting.