FT-IR, GC-MS, and HPLC Profiling of the Bioactive Constituents of Ethyl Acetate Fraction of Eichhornia crassipes as a Hepatoprotectant

Abstract

The study was carried out to analyze the phytoconstituents of the leaves of the aquatic weed E. crassipes.The plant is being traditionally used as a medicinal plant due to its bioactive constituents, but not many reports are available about E.crassipes as a hepatoprotection. The bioactive substances in the ethyl acetate fraction of the hydroethanolic extract of the leaves of E. crassipes (Mart.) Solms were analyzed by using Fourier Transform Infrared Spectroscopy (FT-IR), Gas Chromatography-Mass Spectrometer (GC-MS), and High-Pressure Liquid Chromatography (HPLC). The IR spectrum of ethyl acetate fraction of the hydroethanolic extract shows 16 peaks corresponding to peaks ranging from wave number 3624.42 cm-1 to 610.40 cm-1. In the GC-MS analysis the existence of 3,5-bis(1,1-dimethylethyl)- (C8H12O3), 7 oxabicyclo [4.1.0] heptan-1-ol, acetate(C14H22O), 7,9-di-tert-butyl-1-oxaspiro (4.5) deca-6,9-diene-2,8-dione, palmitic acid vinyl ester (C18H34O2), phytol (C20H40O) as the major constituent of the extract. The extract was further analyzed by HPLC, indicating the presence of phytol as a major constituent that plays a vital role in hepatoprotection. Thus from the results, it is evident that it contains various bioactive compounds and can recommend as a plant of pharmaceutical importance.