AROCLOR 1254 TOXICITY TO MICE LIVER MEMBRANE ATPASES

S. Pathak, R. Kundu
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引用次数: 3

Abstract

Present communication reports the sub-acute dose and exposure duration dependent effects of Aroclor 1254 on total, Na+, K+, Ca++ and Mg++-ATPases of the mouse liver cells. The study tests two hypotheses, (a) whether the sublethal dose or the exposure duration dependent in vivo exposure of hydrophobic PCB (Aroclor 1254) affects the hydrophilic membrane-bound ion dependent ATPases of the liver cells and (b) if a response was observed, whether it was a direct effect of the toxicant on the membrane bound enzymes systems. To check these hypotheses, two groups of mice were subjected to different sublethal oral doses (0.1 and 1 mg kg-1 bw d-1) of Aroclor 1254 for three exposure durations (4, 8 and 12 days). Specific activities of four membrane bound ATPases were estimated from the liver tissue of the aroclor treated mice and compared with a control. Results indicated significant dose as well as duration dependent changes in the enzymatic levels in the hepatic cells of the exposed mice. In most of the cases, the enzyme activity was initially inhibited followed by stimulation after longer exposure duration. The observations suggested that the alteration in the enzyme activity was possibly due to the oxidative stress generated by Aroclor 1254. The results indicated that the membrane bound hydrophilic ATPases were indeed affected by the hydrophobic Aroclor, but the effects were possibly indirect through complex chain of reactions exhibited by the cells.
Aroclor 1254对小鼠肝膜atp酶的毒性
本通讯报道了Aroclor 1254对小鼠肝细胞总atp酶、Na+、K+、Ca++和Mg++- atp酶的亚急性剂量依赖性和暴露时间依赖性作用。该研究检验了两种假设,(a)疏水多氯联苯(Aroclor 1254)的亚致死剂量或暴露时间依赖于体内暴露是否会影响肝细胞的亲水膜结合离子依赖的atp酶;(b)如果观察到反应,这是否是毒物对膜结合酶系统的直接影响。为了验证这些假设,对两组小鼠进行了不同的亚致死口服剂量(0.1和1 mg kg-1 bw -1)的Aroclor 1254,持续3次暴露时间(4、8和12天)。四种膜结合atp酶的特异性活性从aroclor处理小鼠的肝组织估计,并与对照进行比较。结果表明,暴露小鼠肝细胞中酶水平的变化具有明显的剂量依赖性和持续时间依赖性。在大多数情况下,酶活性最初被抑制,然后在较长暴露时间后受到刺激。结果表明,该酶活性的改变可能是由于Aroclor 1254引起的氧化应激所致。结果表明,膜结合的亲水性atp酶确实受到疏水Aroclor的影响,但这种影响可能是通过细胞表现出的复杂连锁反应间接产生的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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