Nguyen Van Man , Hoang Thuy Nguyen , Huynh Thi Phuong Lien , Nguyen Thu Van , Nguyen Kim Giao , Nguyen Minh Lien , Nguyen Thanh Thuy , Irene Dunia , Jean Cohen , E Lucio Benedetti
{"title":"Immunocytochemical characterization of viruses and antigenic macromolecules in viral vaccines","authors":"Nguyen Van Man , Hoang Thuy Nguyen , Huynh Thi Phuong Lien , Nguyen Thu Van , Nguyen Kim Giao , Nguyen Minh Lien , Nguyen Thanh Thuy , Irene Dunia , Jean Cohen , E Lucio Benedetti","doi":"10.1016/S0764-4469(01)01360-9","DOIUrl":null,"url":null,"abstract":"<div><p>Gold immunolabeling combined with negative staining (GINS) provides a valuable immunocytochemical approach that allows a direct ultrastructural definition of all viral vaccine constituents that share common antigenic features with pathogenic viral particles. These results have implications for the development of viral vaccines since it has been demonstrated that incomplete viral particles such as natural empty capsides and Rotavirus-like particles lacking the infective genome are potential candidates for the production of neutralizing antibodies. Furthermore comparative results of the application of GINS to either inactivated vaccines or unfixed samples provide direct evidence that even after inactivation specific antigenic sites are still available for gold immunolabeling.</p></div>","PeriodicalId":100306,"journal":{"name":"Comptes Rendus de l'Académie des Sciences - Series III - Sciences de la Vie","volume":"324 9","pages":"Pages 815-827"},"PeriodicalIF":0.0000,"publicationDate":"2001-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0764-4469(01)01360-9","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comptes Rendus de l'Académie des Sciences - Series III - Sciences de la Vie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0764446901013609","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Gold immunolabeling combined with negative staining (GINS) provides a valuable immunocytochemical approach that allows a direct ultrastructural definition of all viral vaccine constituents that share common antigenic features with pathogenic viral particles. These results have implications for the development of viral vaccines since it has been demonstrated that incomplete viral particles such as natural empty capsides and Rotavirus-like particles lacking the infective genome are potential candidates for the production of neutralizing antibodies. Furthermore comparative results of the application of GINS to either inactivated vaccines or unfixed samples provide direct evidence that even after inactivation specific antigenic sites are still available for gold immunolabeling.