Antioxidant properties of Lactuca sativa leaf extract involved in the protection of biomolecules

Biomedicine & Preventive Nutrition Pub Date : 2013-10-01 DOI:10.1016/j.bionut.2013.06.003

S.N. Harsha , K.R. Anilakumar , M.V. Mithila

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引用次数: 23

Abstract

The aim of the present study was to evaluate the radical scavenging, and biomolecule protective property of Lactuca sativa, an important and commonly used leafy vegetable known for its medicinal properties. The hydro-alcohol extract of Lactuca sativa exhibited strong scavenging effect on 2,2-diphenyl-2-picryl hydrazyl (DPPH), superoxide and nitric oxide, protection against DNA and protein damage. IC ₅₀ values of radical scavenging activity were found to be 162.8 μg/mL for super oxide, 492 μg/mL for nitric oxide radical, 558 μg/mL for metal chelating, and 1236.7 μg/mL for hydroxyl radical. DNA and protein oxidation was prevented dose-dependently and the tail length of DNA as observed in COMET assay was decreased by Lactuca sativa treatment. These results suggest that the extract of Lactuca sativa has potent activity against free radicals and oxidative damage to major biomolecules.

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芥蓝叶提取物抗氧化性能涉及的生物分子保护

摘要本研究的目的是评价莴苣(lacuca sativa)的自由基清除和生物分子保护作用。莴苣是一种重要的、常用的叶菜，因其药用价值而闻名。摘要芥花水醇提取物对2,2-二苯基-2-苦酰肼(DPPH)、超氧化物和一氧化氮具有较强的清除作用，对DNA和蛋白质损伤具有保护作用。清除超氧化物的ic50值为162.8 μg/mL，清除一氧化氮自由基的ic50值为492 μg/mL，清除金属螯合自由基的ic50值为558 μg/mL，清除羟基自由基的ic50值为1236.7 μg/mL。通过剂量依赖性地抑制了DNA和蛋白质的氧化，并减少了DNA的尾长。上述结果表明，芥蓝提取物具有较强的抗自由基活性和抗主要生物分子氧化损伤活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedicine & Preventive Nutrition

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