Preparation of cellulase concoction using differential adsorption phenomenon

Preparative Biochemistry and Biotechnology Pub Date : 2017-01-03 DOI:10.1080/10826068.2016.1275009

S. Birhade, Mukesh Pednekar, Shilpa Sagwal, A. Odaneth, A. Lali

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引用次数: 6

Abstract

ABSTRACT Controlled depolymerization of cellulose is essential for the production of valuable cellooligosaccharides and cellobiose from lignocellulosic biomass. However, enzymatic cellulose hydrolysis involves multiple synergistically acting enzymes, making difficult to control the depolymerization process and generate desired product. This work exploits the varying adsorption properties of the cellulase components to the cellulosic substrate and aims to control the enzyme activity. Cellulase adsorption was favored on pretreated cellulosic biomass as compared to synthetic cellulose. Preferential adsorption of exocellulases was observed over endocellulase, while β-glucosidases remained unadsorbed. Adsorbed enzyme fraction with bound exocellulases when used for hydrolysis generated cellobiose predominantly, while the unadsorbed enzymes in the liquid fraction produced cellooligosaccharides majorly, owing to its high endocellulases activity. Thus, the differential adsorption phenomenon of the cellulase components can be used for the controlling cellulose hydrolysis for the production of an array of sugars.

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利用差动吸附现象制备纤维素酶调合物

纤维素的受控解聚对于从木质纤维素生物质中生产有价值的纤维素低聚糖和纤维素二糖至关重要。然而，酶解纤维素涉及多种协同作用的酶，这使得难以控制解聚过程并产生所需的产物。这项工作利用纤维素酶组分对纤维素底物的不同吸附特性，旨在控制酶的活性。与合成纤维素相比，纤维素酶更有利于预处理纤维素生物质的吸附。胞外酶的吸附优于胞内酶，而β-葡萄糖苷酶则不被吸附。当用于水解时，结合胞外酶的吸附酶部分主要产生纤维素二糖，而液体部分中未吸附的酶主要产生纤维素低聚糖，因为它具有高的胞内酶活性。因此，纤维素酶组分的差异吸附现象可用于控制纤维素水解以生产一系列糖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Preparative Biochemistry and Biotechnology

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