Modulating the lipid profile of blastocyst cell membrane with DPPC multilamellar vesicles

IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2022-06-17 DOI:10.1080/21691401.2022.2088545

Hugo de Rossi, Camila Bortoliero Costa, Luana Teixeira Rodrigues-Rossi, Giovana Barros Nunes, D. Spinosa Chéles, Isabella Maran Pereira, D. F. O. Rocha, E. Feitosa, Ana Valéria Colnaghi Simionato, Gisele Zoccal Mingoti, Pedro Henrique Benites Aoki, M. F. Gouveia Nogueira

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引用次数: 1

Abstract

Abstract The aim of this study was to evaluate the effect of multilamellar vesicles (MLVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in co-culture with in vitro-produced bovine embryos (IVPEs). The stability of five concentrations of MLVs (1.0, 1.25, 1.5, 1.75, and 2.0 mM) produced using ultrapure water or embryonic culture medium with 24 or 48 h of incubation at 38.5 °C with 5% CO2 was assessed. In addition, the toxicity of MLVs and their modulation of the lipid profile of the plasma membrane of IVPEs were evaluated after 48 h of co-culture. Both media allowed the production of MLVs. Incubation (24 and 48 h) did not impair the MLV structure but affected the average diameter. The rate of blastocyst production was not reduced, demonstrating the nontoxicity of the MLVs even at 2.0 mmol/L. The lipid profile of the embryos was different depending on the MLV concentration. In comparison with control embryos, embryos cultured with MLVs at 2.0 mmol/L had a higher relative abundance of six lipid ions (m/z 720.6, 754.9, 759.0, 779.1, 781.2, and 797.3). This study sheds light on a new culture system in which the MLV concentration could change the lipid profile of the embryonic cell membrane in a dose-dependent manner.

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DPPC多层囊泡对囊胚细胞膜脂质分布的调节

摘要本研究旨在评价1,2-二棕榈酰- n-甘油-3-磷脂胆碱(DPPC)与体外培养牛胚胎(IVPEs)共培养对多层囊泡(MLVs)的影响。采用超纯水或胚胎培养基，在38.5°C、5% CO2条件下孵育24或48小时，对5种浓度(1.0、1.25、1.5、1.75和2.0 mM)的mlv的稳定性进行了评估。此外，在共培养48 h后，评估mlv的毒性及其对IVPEs质膜脂质谱的调节。两家媒体都允许制作mlv。孵育(24和48 h)不损害MLV结构，但影响平均直径。即使在2.0 mmol/L的浓度下，mlv的囊胚产生率也没有降低，表明其无毒。MLV浓度不同，胚胎的脂质谱也不同。与对照胚相比，2.0 mmol/L mlv培养的胚具有更高的6种脂质离子相对丰度(m/z为720.6、754.9、759.0、779.1、781.2和797.3)。本研究揭示了一种新的培养系统，其中MLV浓度可以以剂量依赖的方式改变胚胎细胞膜的脂质谱。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL

CiteScore

10.90

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.