Phenolic acid-rich extract of sweet basil restores cholesterol and triglycerides metabolism in high fat diet-fed mice: A comparison with fenofibrate

Hicham Harnafi , Mhamed Ramchoun , Mounique Tits , Jean-Noël Wauters , Michel Frederich , Luc Angenot , Mohammed Aziz , Chakib Alem , Souliman Amrani
{"title":"Phenolic acid-rich extract of sweet basil restores cholesterol and triglycerides metabolism in high fat diet-fed mice: A comparison with fenofibrate","authors":"Hicham Harnafi ,&nbsp;Mhamed Ramchoun ,&nbsp;Mounique Tits ,&nbsp;Jean-Noël Wauters ,&nbsp;Michel Frederich ,&nbsp;Luc Angenot ,&nbsp;Mohammed Aziz ,&nbsp;Chakib Alem ,&nbsp;Souliman Amrani","doi":"10.1016/j.bionut.2013.03.005","DOIUrl":null,"url":null,"abstract":"<div><p>Many spices are often added to foods as additives to enhance organoleptic qualities, such as flavor, aroma and color. Sweet basil (<em>Ocimum basilicum</em> L.) family of Lamiaceae is widely used in cooking for its culinary attributes. In this study, we aimed at the investigation of the hypocholesterolemic and hypotriglyceridemic activities of the basil phenolic acid-rich extract in high fat diet-induced hyperlipemic mice. Hyperlipemia was developed by a high fat diet containing cholesterol, lard and cholic acid. At the beginning of the experiment, animals were divided into three groups, one of them served as normolipidemic control group (NCG), the second hyperlipidemic control group (HCG) and the third basil-treated group (BTG). After 5<!--> <!-->weeks of treatment, basil phenolic acid-rich extract significantly decreased plasma total cholesterol, triglycerides and LDL-cholesterol (−42%,−42% and −86%, respectively, <em>P</em> <!-->&lt;<!--> <!-->0.001). However, HDL-cholesterol was increased (+79%, <em>P</em> <!-->&lt;<!--> <!-->0.001). The extract reduced the atherogenic index and LDL/HDL-C ratio (−88% and −94%, respectively, <em>P</em> <!-->&lt;<!--> <!-->0.001). The reductions of liver total cholesterol and triglycerides were of −50% (<em>P</em> <!-->&lt;<!--> <!-->0.01) and −58% (<em>P</em> <!-->&lt;<!--> <!-->0.01), respectively. The hypolipemic effect of the phenolic acid-rich extract is comparable to that exerted by fenofibrate. This drug significantly reduced plasma total cholesterol, triglycerides and LDL-cholesterol (−25.5%, −51%, and −83.5%, respectively, <em>P</em> <!-->&lt;<!--> <!-->0.001) and increased plasma HDL-cholesterol (+136%, <em>P</em> <!-->&lt;<!--> <!-->0.001). On the other hand, fenofibrate significantly decreased atherogenic index and LDL/HDL-cholesterol ratio (−91% and −93%, respectively, <em>P</em> <!-->&lt;<!--> <!-->0.001). The fenofibrate decreased hepatic total cholesterol by 59.5% and triglycerides by 72%, respectively (<em>P</em> <!-->&lt;<!--> <!-->0.01). HPLC analysis led to identify four major compounds: caftaric acid, cafeic acid, chicoric acid and rosmarinic acid. In conclusion, the Sweet basil contains phenolic products that are able to lower hyperlipidemia and prevent atherosclerosis.</p></div>","PeriodicalId":100182,"journal":{"name":"Biomedicine & Preventive Nutrition","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bionut.2013.03.005","citationCount":"28","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedicine & Preventive Nutrition","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2210523913000172","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 28

Abstract

Many spices are often added to foods as additives to enhance organoleptic qualities, such as flavor, aroma and color. Sweet basil (Ocimum basilicum L.) family of Lamiaceae is widely used in cooking for its culinary attributes. In this study, we aimed at the investigation of the hypocholesterolemic and hypotriglyceridemic activities of the basil phenolic acid-rich extract in high fat diet-induced hyperlipemic mice. Hyperlipemia was developed by a high fat diet containing cholesterol, lard and cholic acid. At the beginning of the experiment, animals were divided into three groups, one of them served as normolipidemic control group (NCG), the second hyperlipidemic control group (HCG) and the third basil-treated group (BTG). After 5 weeks of treatment, basil phenolic acid-rich extract significantly decreased plasma total cholesterol, triglycerides and LDL-cholesterol (−42%,−42% and −86%, respectively, P < 0.001). However, HDL-cholesterol was increased (+79%, P < 0.001). The extract reduced the atherogenic index and LDL/HDL-C ratio (−88% and −94%, respectively, P < 0.001). The reductions of liver total cholesterol and triglycerides were of −50% (P < 0.01) and −58% (P < 0.01), respectively. The hypolipemic effect of the phenolic acid-rich extract is comparable to that exerted by fenofibrate. This drug significantly reduced plasma total cholesterol, triglycerides and LDL-cholesterol (−25.5%, −51%, and −83.5%, respectively, P < 0.001) and increased plasma HDL-cholesterol (+136%, P < 0.001). On the other hand, fenofibrate significantly decreased atherogenic index and LDL/HDL-cholesterol ratio (−91% and −93%, respectively, P < 0.001). The fenofibrate decreased hepatic total cholesterol by 59.5% and triglycerides by 72%, respectively (P < 0.01). HPLC analysis led to identify four major compounds: caftaric acid, cafeic acid, chicoric acid and rosmarinic acid. In conclusion, the Sweet basil contains phenolic products that are able to lower hyperlipidemia and prevent atherosclerosis.

甜罗勒酚酸丰富提取物恢复高脂肪饮食小鼠胆固醇和甘油三酯代谢:与非诺贝特的比较
许多香料通常作为添加剂添加到食品中,以增强感官品质,如风味、香气和颜色。甜罗勒(Ocimum basilicum L.)科因其烹饪特性被广泛用于烹饪。在本研究中,我们旨在研究罗勒酚酸提取物对高脂肪饮食诱导的高脂血症小鼠的降胆固醇和降甘油三酯活性。高脂血症是由含有胆固醇、猪油和胆酸的高脂肪饮食引起的。实验开始时,将动物分为三组,其中一组为降脂对照组(NCG),第二组为高脂血症对照组(HCG),第三组为罗勒处理组(BTG)。治疗5周后,罗勒酚酸提取物显著降低血浆总胆固醇、甘油三酯和低密度脂蛋白胆固醇(分别为- 42%、- 42%和- 86%)。0.001)。然而,高密度脂蛋白胆固醇升高(+79%,P <0.001)。该提取物降低了动脉粥样硬化指数和LDL/HDL-C比值(分别为- 88%和- 94%);0.001)。肝脏总胆固醇和甘油三酯降低- 50% (P <0.01)和- 58% (P <分别为0.01)。富酚酸提取物的降血脂作用与非诺贝特相当。该药物显著降低血浆总胆固醇、甘油三酯和低密度脂蛋白胆固醇(分别为- 25.5%、- 51%和- 83.5%)。0.001)和血浆高密度脂蛋白胆固醇升高(+136%,P <0.001)。另一方面,非诺贝特显著降低动脉粥样硬化指数和LDL/ hdl -胆固醇比值(分别为- 91%和- 93%),P <0.001)。非诺贝特使肝脏总胆固醇降低59.5%,甘油三酯降低72% (P <0.01)。高效液相色谱分析鉴定出四种主要化合物:枸杞子酸、咖啡酸、菊苣酸和迷迭香酸。总之,甜罗勒含有酚类物质,能够降低高脂血症和预防动脉粥样硬化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信