Galactosylation of Branched Cyclodextrins by β-Galactosidases
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Abstract
Transgalactosylated derivatives of branched cyclodextrins (CDs) were synthesized with Bacillus circulars β-galactosidase under the co-existence of lactose and branched CDs. The structure of the transfer products were determined by β-galactosidase digestion, FAB-MS, and 13C-NMR analysis. B. circulans β-galactosidase produced B-galactosyl-(1→4)-α-glucosyl-(1→6)-βCD and β-galactosyl-(1→4)-β-galactosyl-(1→4)-α-glucosyl-(1→6)-1SCD, or β-galactosyl-(1→4)-α-glucosyl-(1→4)-α-glucosyl-(1→6)-CD and β-galactosyl-(1→4)-β-galactosyl-(1→4)-α-glucosyl-(1→4)-α-glucosyl-(1→6)-αCD from the mixture of lactose and glucosyl-BCD or maltosyl-αCD, respectively. Aspergillus oryzae and Penicillium multicolor β-galactosidases also produced transgalactosylated products of branched CDs. These structures are now under investigation.β-半乳糖苷酶对支化环糊精半乳糖基化的影响
在乳糖和支链环糊精共存的条件下，利用环状芽孢杆菌β-半乳糖苷酶合成了支链环糊精(CDs)的转半乳糖基化衍生物。通过β-半乳糖苷酶切、FAB-MS和13C-NMR分析确定了转移产物的结构。B. circulans β-半乳糖苷酶分别从乳糖和葡萄糖基- bcd或麦芽糖基-α cd的混合物中产生b -半乳糖基-(1→4)-α-葡萄糖基-(1→6)-β -半乳糖基-(1→4)-α-葡萄糖基-(1→4)-α-葡萄糖基-(1→4)-α-葡萄糖基-(1→4)-β-半乳糖基-(1→4)-α-葡萄糖基-(1→4)-α-葡萄糖基-(1→6)-α -葡萄糖基-(1→6)-α -乳糖基-(1→4)-α-葡萄糖基-(1→4)-α-葡萄糖基-(1→6)-α -葡萄糖基-(1→6)-α -乳糖基-(1→4)-α-葡萄糖基-(1→4)-α-葡萄糖基-(1→6)-α -葡萄糖基-(1→6)-α -乳糖基-(1→4)-α-葡萄糖基-(1→6)-α -葡萄糖基-(1→6)-α -乳糖基-(1→4)-α-葡萄糖基-(1→4)-α-葡萄糖基-(1→6)-α -葡萄糖基-(1→6)-α -葡萄糖基-(1→6)-α -乳糖基-(1→4)-α-葡萄糖基-(1→4)-α-葡萄糖基-(1→6)-α -葡萄糖基-(1→6)-α -葡萄糖基-(1→6)-α - cd。米曲霉和青霉菌的多色β-半乳糖苷酶也能产生支化cd的转半乳糖基化产物。目前正在对这些建筑物进行调查。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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