Effect of Uremia and Dialysis on "Glucose 6 Phosphate Dehydrogenase Activity"

Abstract

- Glucose 6-phosphatase dehydrogenase (G6PD) is the key enzyme of the hexose monophosphate pathway. In uremic subjects, red blood cells exhibit reduced life spans and are very often the main factor behind the physical activity limitation of the patients. This study was conducted to determine the effect of uremia on GGPD activity and if there is any relationship between the effect of enzyme activity and the number of RBCs by measuring Hb. Also, the study aimed to elucidate the effect of a single dialysis session on G6PD. Glucose 6-phosphate dehydrogenase was studied in (42) patients with end stage disease (ESRD) on regular hemodialysis with (25) healthy individuals (control). The enzyme activity method was assayed according to the procedure mentioned in the text. A single hemodialysis session significantly increased enzyme activity, but the mean value (G6PD) activity remained significantly lower than that of the control. 3-deoxyglucose (accumulated in uremic scrum and erythrocyte) is a potent inhibitor of the G6PD enzyme, and its elimination could participate in the partially corrected G6PD activity after dialysis.