One-step pre-column derivatization method for HPLC-UV determination of organic acids in fruit juices

IF 1 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-06-28 DOI:10.1080/10826076.2023.2227887

Nada Seddiq, A. Asan

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Abstract

Abstract A simple and effective one-step pre-column derivatization method for reversed-phase HPLC-UV determination of the major organic acids (citric, malic, tartaric, and succinic acids) in fruit juices has been developed. The method is based on the coupling reaction of organic acids with 1-naphthylamine in the presence of N,N′-diisopropylcarbodiimide. The resulting organic acid amide derivatives were successfully separated on an AQ-C18 column and detected by a UV detector at 222 nm. Isocratic elution was performed using an acetonitrile-methanol-tetrahydrofuran-water (37:4:1:58; v/v) mixture as a mobile phase at a flow rate of 2.0 mL/min. Validation studies showed good recoveries ranging from 88.97 to 92.52% with RSD % between 2.95 and 4.56%. The detection limits obtained were 0.18, 0.48, 0.14, and 0.69 µg/mL for tartaric acid, malic acid, succinic acid, and citric acid, respectively. The method was successfully applied to three different commercial fruit juice and nectar samples with little or no clean-up procedures. Graphical Abstract

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一步柱前衍生化法测定果汁中有机酸

建立了反相HPLC-UV法测定果汁中主要有机酸(柠檬酸、苹果酸、酒石酸和琥珀酸)的一步柱前衍生法。该方法是基于有机酸与1-萘胺在N,N ' -二异丙基碳二亚胺存在下的偶联反应。得到的有机酸酰胺衍生物在AQ-C18色谱柱上成功分离，并在222 nm处用紫外检测器检测。用乙腈-甲醇-四氢呋喃-水(37:4:1:58;v/v)混合物作为流动相，流速为2.0 mL/min。验证试验表明，加样回收率为88.97 ~ 92.52%，RSD %为2.95 ~ 4.56%。酒石酸、苹果酸、琥珀酸和柠檬酸的检出限分别为0.18、0.48、0.14和0.69µg/mL。该方法成功地应用于三种不同的商业果汁和花蜜样品，很少或没有清理程序。图形抽象

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来源期刊

Journal of Liquid Chromatography & Related Technologies 化学-分析化学

CiteScore

2.80

自引率

0.00%

发文量

审稿时长

4.9 months

期刊介绍： The Journal of Liquid Chromatography & Related Technologies is an internationally acclaimed forum for fast publication of critical, peer reviewed manuscripts dealing with analytical, preparative and process scale liquid chromatography and all of its related technologies, including TLC, capillary electrophoresis, capillary electrochromatography, supercritical fluid chromatography and extraction, field-flow technologies, affinity, and much more. New separation methodologies are added when they are developed. Papers dealing with research and development results, as well as critical reviews of important technologies, are published in the Journal.