Expression and distribution of Nob1 in the developing rat cochleae

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns Pub Date : 2022-06-01 DOI:10.1016/j.gep.2022.119235

Xiaogang An , Ye Wang , Bang Han , Liu Hong , Dingjun Zha , Yu Han

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引用次数: 0

Abstract

Background

Mammalian inner ear cells are produced during embryonic development. NIN1/RPN12 binding protein 1 homolog (Nob1), a ribosome assembly factor, is believed to be involved in transcriptional regulation and may play an important role in mediating certain physiological and pathological functions.

Methods

We investigated the expression levels and distribution patterns of Nob1 in rat cochlea at different developmental stages using reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis, and immunofluorescence microscopy (P0, P3, P7, P14, and P30, n = 11 per age group).

Results

The expression of Nob1 was gradually upregulated from Postnatal day (P) 0 to P14 and stabilized at P30. Our results analyzing Nob1 expression at P0 and P3 in the rat cochleae revealed expression in the greater epithelial ridge cells, lesser epithelial ridge cells, and spiral ganglion neurons (SGNs), whereas low levels of Nob1 expression were observed in the stria vascularis cells. During the development of the cochlea, the organ of Corti undergoes dramatic architectural remodeling. Nob1 expression was stably detected in supporting cells and hair cells from P7 to P30, and its expression gradually increased in the SGNs.

Conclusions

Thus, the present study revealed that upregulation of Nob1 might explain the maturation of the cytoarchitecture in the cochlea during the postnatal period, and a complete understanding of its function in the mammalian inner ear may help to develop strategies to resolve auditory hair cell loss.

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Nob1在发育中的大鼠耳蜗的表达和分布

哺乳动物内耳细胞是在胚胎发育过程中产生的。NIN1/RPN12结合蛋白1同源物(binding protein 1 homolog, Nob1)是一种核糖体组装因子，被认为参与转录调控，可能在介导某些生理和病理功能中发挥重要作用。方法采用逆转录聚合酶链反应(RT-PCR)、Western blot和免疫荧光显微镜观察不同发育阶段大鼠耳蜗中Nob1的表达水平和分布规律(P0、P3、P7、P14和P30，每年龄组n = 11)。结果Nob1的表达在出生后0 ~ 14天逐渐上调，在30天稳定表达。我们分析了大鼠耳蜗P0和P3的Nob1表达，结果显示Nob1在大上皮脊细胞、小上皮脊细胞和螺旋神经节神经元(sgn)中表达，而在血管纹细胞中低水平表达。在耳蜗的发育过程中，耳蜗器官经历了剧烈的结构重塑。从P7到P30，在支持细胞和毛细胞中稳定检测到Nob1的表达，其在sgn中的表达逐渐增加。因此，本研究揭示了Nob1的上调可能解释了出生后耳蜗细胞结构的成熟，并且对其在哺乳动物内耳中的功能的全面了解可能有助于制定解决听觉毛细胞丢失的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Expression Patterns 生物-发育生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include: -In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression -Temporal studies of large gene sets during development -Transgenic studies to study cell lineage in tissue formation