Screening of Streptococcus Suis serotype 2 resistance genes in mice with SNP and transcriptomic microarray

Hongjie Fan, Hong Zhou, Xiaomeng Pei
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引用次数: 3

Abstract

A bacterial strain Bacillus spp. ETL-1949 with remarkable ability to decolourize the reactive azo dyes such as reactive red, reactive yellow, reactive brilliant red and reactive brilliant blue was isolated from the textile effluent contaminated site. The effluent sample was collected from local textile industry, Ankleshwar, Gujarat, India Static conditions with 10 g/l glucose, pH 9, 37°C, 20% inoculum concentration, 50 mg/l of dye concentration, 3 g/l of NH4NO3, were considered to be the optimum decolourizing conditions. Bacillus spp. ETL-1949 grew well in these optimum conditions, resulting in 82% decolourization extent 7 days of incubation. Phenotypic characterization and phylogenetic analysis of the 16S rRNA sequence indicated that the bacterial strain belonged to the genus Bacillus. UV (Ultra Violet) analysis of bacterial isolate suggested that it exhibited decolourizing activity, rather than inactive surface adsorption. This decolourization extent and facile conditions show the potential for this bacterial strain to be used in the biological treatment of textile effluent or dyes.
用SNP和转录组芯片筛选小鼠猪链球菌血清2型耐药基因
从纺织废水污染现场分离到一株对活性红、活性黄、活性艳红、活性艳蓝等偶氮染料具有显著脱色能力的芽孢杆菌ETL-1949。废水采集自印度古吉拉特邦Ankleshwar当地纺织工业,静态条件为葡萄糖10 g/l, pH 9, 37℃,接种量20%,染料浓度50 mg/l, NH4NO3 3 g/l。Bacillus spp. ETL-1949在此条件下生长良好,培养7 d脱色率达82%。表型鉴定和16S rRNA序列的系统发育分析表明该菌株属于芽孢杆菌属。紫外(UV)分析表明,细菌分离物表现出脱色活性,而不是无活性的表面吸附。这种脱色程度和易脱色的条件表明,该菌株有潜力用于纺织废水或染料的生物处理。
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