APPLICATION OF BIOLUMINESCENT METHODS TO STUDY STRESS EFFECTS ON LIVING SYSTEMS

Abstract

Bioluminescent methods based on firefly luciferase for detecting the effect of membrane-active agents (antibiotics and saponins) on living prokaryotic and eukaryotic cells have been developed. New data on the kinetics and mechanism of action of colistin on E. coli cells and digitonin cells on NEK293 cells were obtained. Examples of the use of constitutive and inducible luciferase reporters in cell biology are given.