Fereshte Abdolmaleki, S. Moghbelinejad, Hossein Ahmadpour-Yazdi
{"title":"The Effect of Myricetin Flavonoid on the Expression of Fyn Gene in Melanoma Cells (A375)","authors":"Fereshte Abdolmaleki, S. Moghbelinejad, Hossein Ahmadpour-Yazdi","doi":"10.5812/BHS.14484","DOIUrl":null,"url":null,"abstract":"Background: Malignant melanoma as one of the most common cancers is currently spreading worldwide. Regarding after-effect \nof advanced treatments, using natural products has attracted much attention. Flavonoids, polyphenol compounds rich in diet, are \nbeing considered for their therapeutic preventive features. Fyn gene, a member of the protein tyrosine kinase oncogene family, has \nbecome an important target for therapy goals. \nObjectives: The aim of this study was to assess Fyn gene expression after treatment of melanoma cells with myricetin. \nMethods: In this study, the melanoma cells were treated with different concentrations of myricetin (0 to 100 �M) and their viability \nwas determined by the methylthiazolyl diphenyl-tetrazolium bromide (MTT) assay, also the expression of Fyn gene in treated \ncells with selected concentrations of myricetin (0, 20, 40, 50, and 60 �M) was detected by real time quantitative polymerase chain \nreaction (qPCR). \nResults: The current investigation showed that treatment of A375 melanoma cells with the dietary flavonoid myricetin (3, 5, 7- \ntrihydroxy-2-(3, 4, 5,-trihydroxy phenyl)-4- chromenone), resulted in decreased cell viability and increased expression of Fyn gene. \nThe MTT assay analysis of exposed cells with different concentrations of myricetin showed that up to 25 �Mof myricetin had no \ncytotoxicity effect on A375 cells, also with increasing of myricetin concentration, the repression of cell proliferation developed as \nwell. \nConclusions: Real time qPCR analysis of Fyn expression in exposed cells with various concentration of myricetin leads to overexpression \nof this gene, dose dependently. Through this research, it was determined that myricetin with its anti-proliferative potential \ncould suppress the development of cancer cells. On the other hand, since Fyn kinase could be involved in tumorigenesis of some \ncancer cells, it could be concluded that myricetin could effect the carcinogenicity of Fyn function in melanoma cells. \nKeywords: Melanoma, A375, Myricetin, Fyn Gene","PeriodicalId":8849,"journal":{"name":"Biotechnology and Health Sciences","volume":"21 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2017-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology and Health Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5812/BHS.14484","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Malignant melanoma as one of the most common cancers is currently spreading worldwide. Regarding after-effect
of advanced treatments, using natural products has attracted much attention. Flavonoids, polyphenol compounds rich in diet, are
being considered for their therapeutic preventive features. Fyn gene, a member of the protein tyrosine kinase oncogene family, has
become an important target for therapy goals.
Objectives: The aim of this study was to assess Fyn gene expression after treatment of melanoma cells with myricetin.
Methods: In this study, the melanoma cells were treated with different concentrations of myricetin (0 to 100 �M) and their viability
was determined by the methylthiazolyl diphenyl-tetrazolium bromide (MTT) assay, also the expression of Fyn gene in treated
cells with selected concentrations of myricetin (0, 20, 40, 50, and 60 �M) was detected by real time quantitative polymerase chain
reaction (qPCR).
Results: The current investigation showed that treatment of A375 melanoma cells with the dietary flavonoid myricetin (3, 5, 7-
trihydroxy-2-(3, 4, 5,-trihydroxy phenyl)-4- chromenone), resulted in decreased cell viability and increased expression of Fyn gene.
The MTT assay analysis of exposed cells with different concentrations of myricetin showed that up to 25 �Mof myricetin had no
cytotoxicity effect on A375 cells, also with increasing of myricetin concentration, the repression of cell proliferation developed as
well.
Conclusions: Real time qPCR analysis of Fyn expression in exposed cells with various concentration of myricetin leads to overexpression
of this gene, dose dependently. Through this research, it was determined that myricetin with its anti-proliferative potential
could suppress the development of cancer cells. On the other hand, since Fyn kinase could be involved in tumorigenesis of some
cancer cells, it could be concluded that myricetin could effect the carcinogenicity of Fyn function in melanoma cells.
Keywords: Melanoma, A375, Myricetin, Fyn Gene