The Hordeum bulbosum 25S-18S rDNA region: comparison with Hordeum vulgare and other Triticeae

O. Georgiev, K. Mishev, M. Krasnikova, M. Kitanova, A. Dimitrova, L. Karagyozov
{"title":"The Hordeum bulbosum 25S-18S rDNA region: comparison with Hordeum vulgare and other Triticeae","authors":"O. Georgiev, K. Mishev, M. Krasnikova, M. Kitanova, A. Dimitrova, L. Karagyozov","doi":"10.1515/znc-2018-0109","DOIUrl":null,"url":null,"abstract":"Abstract Hordeum vulgare and Hordeum bulbosum are two closely related barley species, which share a common H genome. H. vulgare has two nucleolar organizer regions (NORs), while the NOR of H. bulbosum is only one. We sequenced the 2.5 kb 25S-18S region in the rDNA of H. bulbosum and compared it to the same region in H. vulgare as well as to the other Triticeae. The region includes an intergenic spacer (IGS) with a number of subrepeats, a promoter, and an external transcribed spacer (5′ETS). The IGS of H. bulbosum downstream of 25S rRNA contains two 143-bp repeats and six 128-bp repeats. In contrast, the IGS in H. vulgare contains an array of seven 79-bp repeats and a varying number of 135-bp repeats. The 135-bp repeats in H. vulgare and the 128-bp repeats in H. bulbosum show similarity. Compared to H. vulgare, the 5′ETS of H. bulbosum is shorter. Additionally, the 5′ETS regions in H. bulbosum and H. vulgare diverged faster than in other Triticeae genera. Alignment of the Triticeae promoter sequences suggests that in Hordeum, as in diploid Triticum, transcription starts with guanine and not with adenine as it is in many other plants.","PeriodicalId":23894,"journal":{"name":"Zeitschrift für Naturforschung C","volume":"22 1","pages":"319 - 328"},"PeriodicalIF":0.0000,"publicationDate":"2019-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift für Naturforschung C","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/znc-2018-0109","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

Abstract

Abstract Hordeum vulgare and Hordeum bulbosum are two closely related barley species, which share a common H genome. H. vulgare has two nucleolar organizer regions (NORs), while the NOR of H. bulbosum is only one. We sequenced the 2.5 kb 25S-18S region in the rDNA of H. bulbosum and compared it to the same region in H. vulgare as well as to the other Triticeae. The region includes an intergenic spacer (IGS) with a number of subrepeats, a promoter, and an external transcribed spacer (5′ETS). The IGS of H. bulbosum downstream of 25S rRNA contains two 143-bp repeats and six 128-bp repeats. In contrast, the IGS in H. vulgare contains an array of seven 79-bp repeats and a varying number of 135-bp repeats. The 135-bp repeats in H. vulgare and the 128-bp repeats in H. bulbosum show similarity. Compared to H. vulgare, the 5′ETS of H. bulbosum is shorter. Additionally, the 5′ETS regions in H. bulbosum and H. vulgare diverged faster than in other Triticeae genera. Alignment of the Triticeae promoter sequences suggests that in Hordeum, as in diploid Triticum, transcription starts with guanine and not with adenine as it is in many other plants.
球球Hordeum 25S-18S rDNA区:与普通Hordeum及其他小麦科的比较
摘要大麦Hordeum vulgare和大麦Hordeum bulbosum是两个亲缘关系较近的大麦品种,它们具有共同的H基因组。黄茎有两个核仁组织区,而黄茎只有一个核仁组织区。我们对球芽草rDNA中2.5 kb的25S-18S区域进行了测序,并将其与普通小麦和其他小麦科植物的相同区域进行了比较。该区域包括一个带有多个亚重复序列的基因间间隔段(IGS)、一个启动子和一个外部转录间隔段(5'ETS)。球芽草25S rRNA下游的IGS包含2个143-bp重复序列和6个128-bp重复序列。相比之下,H. vulgare的IGS包含7个79-bp的重复序列和不同数量的135-bp重复序列。黄貂草的135-bp重复序列与黄貂草的128-bp重复序列具有相似性。与普通黄茎相比,黄茎黄茎的5′ets较短。此外,小麦和普通小麦的5′ets区分化速度快于其他小麦属。小麦启动子序列的比对表明,在Hordeum中,与二倍体小麦一样,转录从鸟嘌呤开始,而不是像在许多其他植物中那样从腺嘌呤开始。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信