Correlations of Interleukin-6 (IL-6) ( G174C and G572C ) gene polymorphisms and sera levels with risk of Coronary Artery Disease in Thi – Qar province

Taqwa Kadhem, R. Salih
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Abstract

        The present study was carried out in the labs of Technical Institute –Al-Nasiriyah /Community Health Department and Nasiriyah Heart Center in Thi- Qar province, during the period of research  was extended from January to July 2022 . The aim of study was to determination polymorphisms of IL-6 gene in patients with Coronary Artery Disease and measuring its levels in serum using a technique enzyme-linked immune sorbent adsorptive  (ELISA). The study included a total of 100 Iraqi patients with coronary artery disease(CAD) and healthy control group their age between 3-85 years.  DNA has been isolated and PCR was performed by using primers specific for  genotypes of IL-6 gene  (572 C/G   and 174 C/G), the results showed that gel electrophoresis for amplified IL-6 gene of CAD patients, gel electrophoresis of 167bp (IL-6 572C/G) PCR products and 198bp PCR product(IL-6 174C/G). Frequency of genotypes for the IL6 gene showed a high frequency of GG genotype compared to the GC and CC genotypes in all study groups , the frequency of G and C allele in IL6 gene was 95 % and 5 %, respectively with significant differences between genotypes in various groups, in the patient group (90 %) had GC genotype, (10%) were heterozygote and had (0%) had CC genotype compare with control group, (100%) had GC genotype, (0%) were heterozygote and had (0%) had CC genotype. The immunological study showed that there was a significant increase of concentrations of (IL-6) in patients compared to the healthy  control group. Thus it seems There is no correlation between IL-6 gene polymorphisms and the risk of coronary artery disease in Thi- Qar province and the Serological Assay (ELISA) showed a significant increase in the concentration of IL-6  in patients with coronary artery disease compared with the healthy control group.
白介素-6 (IL-6) (G174C和G572C)基因多态性和血清水平与Thi Qar省冠状动脉疾病风险的相关性
本研究是在Thi- Qar省纳西里耶技术学院/社区卫生部门和纳西里耶心脏中心的实验室进行的,研究期间从2022年1月延长至7月。研究目的是利用酶联免疫吸附(ELISA)技术测定冠心病患者血清中IL-6基因多态性并测定其水平。研究对象为100名年龄在3-85岁之间的伊拉克冠心病患者和健康对照组。利用IL-6基因型(572 C/G和174C/G)特异性引物分离DNA,进行PCR检测,结果显示CAD患者IL-6基因扩增产物凝胶电泳为167bp (IL-6 572C/G) PCR产物和198bp (IL-6 174C/G) PCR产物。白细胞介素6基因的基因型频率显示高频GG基因型相比,GC和CC基因型在所有学习小组,在白细胞介素6基因G和C等位基因的频率为95%和5%,分别与基因型在不同群体之间的显著差异,病人组(90%)GC基因型,(10%)是杂合子,有(0%)CC基因型与对照组相比,GC基因型(100%),(0%)杂合子,CC基因型(0%)。免疫学研究表明,与健康对照组相比,患者体内(IL-6)浓度显著升高。因此,IL-6基因多态性与Thi- Qar省冠状动脉疾病的风险之间没有相关性,血清学分析(ELISA)显示冠状动脉疾病患者的IL-6浓度与健康对照组相比显着增加。
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