Ochratoxin A – Bestimmung von Ochratoxin A in Serum mittels CE-LIF. Biomonitoring-Methode

The MAK-Collection for Occupational Health and Safety Pub Date : 2020-01-01 DOI:10.34865/BI30347D5_4OR

A. Hartwig, Michael Arand

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Abstract

The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed the presented biomonitoring method. The method enables the determination of ochratoxin A in small volumes of serum. The samples are purified by liquid-liquid extraction and the analyte is extracted from the acidified sample with dichloromethane. After the extraction agent has been removed, the residue is dissolved in water. The extracts are injected into the capillary, then the individual components are separated by capillary electrophoresis and detected by laser-induced fluorescence. Coumarin-3-carboxylic acid is used as an internal standard. Calibration standards are prepared in serum and processed in the same way as the samples to be analysed. The MAK Collection for Occupational Health and Safety 2020, Vol 5, No 4 1 Biomonitoring-Methoden – Ochratoxin A in Serum 1 Kenndaten der Methode Matrix Serum Analytisches Messprinzip Kapillarelektrophorese mit laserinduzierter Fluoreszenzdetektion (CE-LIF) Parameter und entsprechender Arbeitsstoff Arbeitsstoff CAS-Nr. Parameter CAS-Nr. Ochratoxin A 303-47‐9 Ochratoxin A 303-47‐9 Zuverlässigkeitskriterien

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och有毒A透过serf确定血清中的och有毒ABiomonitoring-Methode

调查工作区内化合物对健康危害的参议院常设委员会的"生物材料分析"工作组制定了所提出的生物监测方法。本方法可用于小体积血清中赭曲霉毒素A的测定。用液-液萃取法纯化样品，用二氯甲烷从酸化样品中提取分析物。萃取剂除去后，将残留物溶解在水中。将提取液注入毛细管，毛细管电泳分离各组分，激光诱导荧光检测。香豆素-3-羧酸作为内标。校准标准品在血清中制备，处理方法与待分析样品相同。中华职业健康与安全杂志，2020,Vol . 5, No . 4 1生物监测方法-血清中赭曲霉毒素A的含量测定方法-基质血清分析方法(Messprinzip kapillarektrophrese - lif)参数CAS-Nr。赭曲霉毒素A 303-47‐9 Zuverlässigkeitskriterien

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The MAK-Collection for Occupational Health and Safety

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