Effect of inorganic salts on bacterial omega-3 PUFA production

Ahmed Abd Elrazak, A. Ward, J. Glassey
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引用次数: 2

Abstract

ABSTRACT The increasing demand of omega-3 in the market and the challenges facing its conventional supplies led to an increasing interest to microbial omega-3 sources. This research concentrates on the statistical role of some metal ions on the biosynthesis and productivity of eicosapentaenoic acid (essential omega-3 element) in bacterial isolate, Shewanella 717. A Plackett–Burman design was applied to screen the main effect of all metal salts entrenched in the artificial sea water medium components. Four salts, in particular, in addition to the interaction among them were highlighted as having a statistically significant effect upon the growth and/or eicosapentaenoic acid production. A subsequent central composite design was performed to determine the exact optimum concentration of each of the chosen variables which was found to be 2.5, 1.8, 1.2, and 23 g/l, for Na2HPO4, MgSO4, KCl, and NaCl, respectively. All the experiments were performed with the minimal amount of carbon and nitrogen to eliminate any potential masking effect. A bioreactor batch run was operated and the ion uptake was monitored, using EDAX® electron microscopy, concluding that the process of microbial omega-3 production could be a phosphate-limited process. Optimizing the concentration of the tested metal ions led to a remarkable increase in the omega-3 productivity resulted in a 30, 9, and 10 times increase in yield, concentration, and percentage to the total fatty acids, respectively, even though the carbon and nitrogen were kept constant all over the research work.
无机盐对细菌omega-3 PUFA生成的影响
欧米伽-3在市场上的需求不断增加,其传统供应面临的挑战导致了对微生物欧米伽-3来源的兴趣日益增加。本研究主要研究了一些金属离子对分离的希瓦氏菌717中二十碳五烯酸(必需的ω -3元素)的生物合成和生产力的统计作用。采用Plackett-Burman设计筛选人工海水介质组分中所有金属盐的主要影响。特别是四种盐,除了它们之间的相互作用外,被强调为对生长和/或二十碳五烯酸产生统计上显著的影响。对Na2HPO4、MgSO4、KCl和NaCl的最佳浓度分别为2.5、1.8、1.2和23 g/l,随后进行了中心复合设计以确定每个选定变量的确切最佳浓度。所有的实验都是在最少量的碳和氮的情况下进行的,以消除任何潜在的掩蔽效应。采用EDAX®电子显微镜对生物反应器进行了批量运行,并对离子摄取进行了监测,得出结论:微生物生产omega-3的过程可能是一个磷酸盐限制的过程。优化被测金属离子的浓度导致omega-3生产力的显著提高,即使在整个研究工作中碳和氮保持不变,其产量、浓度和占总脂肪酸的百分比分别增加了30倍、9倍和10倍。
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