The expression of TRAIL and its receptors in osteosarcoma cells and the apoptosis effect of a combination of TRAIL, adriamycin and IFN-γ on MG-63 cells

Journal of Nanjing Medical University Pub Date : 2009-07-01 DOI:10.1016/S1007-4376(09)60065-4

Chao Deng, Zengwu Shao, Xiaoqian Xiong, Zhichuan Liu, Zhicai Zhang

{"title":"The expression of TRAIL and its receptors in osteosarcoma cells and the apoptosis effect of a combination of TRAIL, adriamycin and IFN-γ on MG-63 cells","authors":"Chao Deng, Zengwu Shao, Xiaoqian Xiong, Zhichuan Liu, Zhicai Zhang","doi":"10.1016/S1007-4376(09)60065-4","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>This study investigated the effects of rh-TRAIL with or without chemotherapeutic drugs on the apoptosis of the osteosarcoma cell line, MG-63, and the influence of chemotherapeutic drugs on changes in the expression of DR5 and Yin Yang1(YY1) in MG- 63 cells.</p></div><div><h3>Methods</h3><p>The effects of treatment with rh-TRAIL alone and/or chemotherapeutic drugs on MG-63 cell growth inhibition and apoptosis were measured using the MTT assay, FACS analysis of Annexin V labeled cells, and the mRNA changes of DR5 and YY1 were detected by RT-PCR.</p></div><div><h3>Results</h3><p>Rh-TRAIL protein inhibited the growth of MG-63 cells, and this inhibition was increased by adriamycin and IFN-γ. Adriamycin and IFN-γ significantly facilitated the induction of the expression of DR5 and reduced the expression of YY1.</p></div><div><h3>Conclusion</h3><p>The apoptosis-inducing effect of rh-TRAIL in MG-63 cells was enhanced by chemotherapeutic drugs.</p></div>","PeriodicalId":100807,"journal":{"name":"Journal of Nanjing Medical University","volume":"23 4","pages":"Pages 251-256"},"PeriodicalIF":0.0000,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1007-4376(09)60065-4","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nanjing Medical University","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1007437609600654","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 1

Abstract

Objective

This study investigated the effects of rh-TRAIL with or without chemotherapeutic drugs on the apoptosis of the osteosarcoma cell line, MG-63, and the influence of chemotherapeutic drugs on changes in the expression of DR5 and Yin Yang1(YY1) in MG- 63 cells.

Methods

The effects of treatment with rh-TRAIL alone and/or chemotherapeutic drugs on MG-63 cell growth inhibition and apoptosis were measured using the MTT assay, FACS analysis of Annexin V labeled cells, and the mRNA changes of DR5 and YY1 were detected by RT-PCR.

Results

Rh-TRAIL protein inhibited the growth of MG-63 cells, and this inhibition was increased by adriamycin and IFN-γ. Adriamycin and IFN-γ significantly facilitated the induction of the expression of DR5 and reduced the expression of YY1.

Conclusion

The apoptosis-inducing effect of rh-TRAIL in MG-63 cells was enhanced by chemotherapeutic drugs.

查看原文本刊更多论文

TRAIL及其受体在骨肉瘤细胞中的表达及TRAIL、阿霉素和IFN-γ联合作用对MG-63细胞凋亡的影响

目的研究rh-TRAIL联合或不联合化疗药物对MG-63骨肉瘤细胞系凋亡的影响，以及化疗药物对MG-63细胞DR5和阴阳1(YY1)表达变化的影响。方法采用MTT法、Annexin V标记细胞FACS法检测rh-TRAIL和/或化疗药物治疗对MG-63细胞生长抑制和凋亡的影响，RT-PCR检测DR5和YY1 mRNA的变化。结果rh - trail蛋白对MG-63细胞的生长有抑制作用，阿霉素和IFN-γ可增强这种抑制作用。阿霉素和IFN-γ显著促进DR5的表达诱导，降低YY1的表达。结论化疗药物可增强rh-TRAIL对MG-63细胞的诱导凋亡作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Nanjing Medical University

自引率

0.00%

发文量