Ceramide regulation on sensitivity of common carotid arteries of the simulated weightless rats

中华航空航天医学杂志 Pub Date : 2016-06-15 DOI:10.3760/CMA.J.ISSN.1007-6239.2016.02.013

Yaoping Cheng, Yuting Su, Xingxing Meng, Hai-Jun Zhang, Xiao-ping Xie, Yao-ming Chang

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Abstract

Objective To investigate the sensitivity of common carotid arteries of the rats to ceramide (Cer) regulation after simulated weightlessness. Methods Fourty-eight male Sprague-Dawley rats that matched for weight were randomly assigned to suspended group (n=24) and control group (n=24). Tail suspension of rats was carried out for 4 wk to simulate the effect of weightlessness. Isometric force recording system was used to detect the vasoconstriction and vasodilation response of common carotid arteries ring. Dihydroethidium fluorescent probe was used to evaluate the level of superoxide anion (O2-· ) in arteries. Results Vasoconstriction response and vasodilation response showed insignificant difference between suspended group and control group. Incubation with C6-ceramide (C6-Cer) or Cer antibody (anti-Cer) did not change the vasoconstriction response and O2-· level of CA in control group but increased Cer level, vasoconstriction response to KCl and O2-· level of common carotid arteries in control group, and did not change the vasodilation response of common carotid arteries both in control and suspended group. In suspended group, C6-Cer incubation increased vasoconstriction response of common carotid arteries both to potassium chloride and phenylephrine, as well as the O2-· level (t=6.077-12.630, P<0.05) but less to acetylcholine and nitroprusside sodium. The anti-Cer incubation decreased response vasoconstriction of common carotid arteries in suspended both to potassium chloride and phenylephrine, as well as the O2-·level (t=11.582-32.130, P<0.05) but the vasodilation response. The incubation of superoxide dismutase or hydrogen peroxide depressed the changes of vasoconstriction response caused by C6-Cer or anti-Cer. Conclusions Cer regulation increases the sensitivity of common carotid arterial constriction in the simulated weightless rats and the mechanism is concerned with reactive oxygen level. Key words: Weightlessness simulation; Carotid artery, common; Phospholipases; Ceramide; Oxidative stress; Rats, Sprague-Dawley

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神经酰胺对模拟失重大鼠颈总动脉敏感性的调节

目的探讨模拟失重后大鼠颈总动脉对神经酰胺调节的敏感性。方法48只体重匹配的雄性Sprague-Dawley大鼠随机分为悬浮组(n=24)和对照组(n=24)。采用大鼠尾部悬吊4周模拟失重效果。采用等距力记录系统检测颈总动脉环血管收缩和血管舒张反应。采用双氢乙锭荧光探针检测大鼠动脉超氧阴离子(O2-·)水平。结果悬吊组与对照组血管收缩反应、血管舒张反应差异无统计学意义。c6 -神经酰胺(C6-Cer)或Cer抗体(抗Cer)孵育未改变对照组CA的血管收缩反应和O2-·水平，但提高了对照组颈总动脉的Cer水平、血管对KCl的收缩反应和O2-·水平，对照组和悬吊组均未改变颈总动脉的血管舒张反应。悬吊组颈总动脉对氯化钾和苯肾上腺素的血管收缩反应及O2-·水平升高(t=6.077 ~ 12.630, P<0.05)，对乙酰胆碱和硝普钠的收缩反应较低。抗癌孵育降低了悬液大鼠颈总动脉对氯化钾和苯肾上腺素的血管收缩反应及O2-·水平(t=11.582 ~ 32.130, P<0.05)，但降低了血管舒张反应。超氧化物歧化酶或过氧化氢孵育可抑制C6-Cer或抗- cer引起的血管收缩反应的变化。结论Cer调节增加了模拟失重大鼠颈总动脉收缩的敏感性，其机制与活性氧水平有关。关键词:失重模拟;颈动脉，普通;磷脂酶;神经酰胺;氧化应激;老鼠,Sprague-Dawley

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中华航空航天医学杂志航空航天医学

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2962

期刊介绍： The aim of Chinese Journal of Aerospace Medicine is to combine theory and practice, improve and popularize, actively advocate a hundred flowers bloom and a hundred schools of thought contend, advocate seeking truth from facts, promote the development of the related disciplines of aerospace medicine and human efficiency, and promote the exchange and penetration of aerospace medicine and human efficiency with other biomedical and engineering specialties. Topics of interest for Chinese Journal of Aerospace Medicine include: -The content of the journal belongs to the discipline of special medicine and military medicine, with the characteristics of multidisciplinary synthesis and cross-penetration, and mainly reflected in the aerospace industry, aerospace flight safety and efficiency, as well as the synthesis of special medicine, preventive medicine, environmental medicine, psychology, etc. -Military aeromedicine (Air Force, Navy and Army aeromedicine) and civil aeromedicine, with a balance of aerospace medicine are the strengths of the journal. -The change in aerospace medicine from a focus on promoting physiological compensatory adaptations to enhancing human performance under extreme environmental conditions is what the journal is helping to promote. -The expansion of manuscripts in high altitude medicine is also a special emphasis of the journal.