The Potency of Bacillus siamensis LDR as Biocontrol Agent Against Fungal Phytopathogen

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI:10.2991/absr.k.210621.079

I. Santoso, Q. G. Fadhilah, A. E. Maryanto, Yasman

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引用次数: 2

Abstract

Bacillus spp. was recognized as biocontrol agent because could produce antifungal towards many phytopathogenic fungi. In relation to study the potency of B. siamensis LDR as biocontrol, antagonistic activity of the bacilli was evaluated against Fusarium sp., Ganoderma sp., and Chaetomium globosum InaCC F228. The antagonist assay was carried out by dual culture method using streak and pour plate technique. Result of antagonistic activity assay with streak technique showed that growth inhibition rate of Fusarium sp., Ganoderma sp., and Chaetomium globosum InaCC F228 were 36.83%, 55.20%, and 33.78%, respectively. Antagonist assay using pour plate technique showed that percentage growth of inhibition for Fusarium sp. (93.96%), Ganoderma sp. (100%), and Chaetomium globosum InaCC F228 (76.64%) were higher than the results from streak method. Furthermore, results from antibiosis assay showed that growth inhibition rate of Fusarium sp., Ganoderma sp., and Chaetomium globosum InaCC F228 were 10.85%, 73.31%, and 30.32% respectively.

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暹罗芽孢杆菌LDR对真菌植物病原菌的防效研究

芽孢杆菌对许多植物病原真菌具有抗真菌作用，是公认的生物防治剂。为了研究结核分枝杆菌的生物防治效果，测定了结核分枝杆菌对镰刀菌、灵芝菌和球毛菌InaCC F228的拮抗活性。拮抗试验采用条纹法和平板法双重培养。条纹法测定病原菌对镰刀菌、灵芝菌和球毛菌InaCC F228的抑制率分别为36.83%、55.20%和33.78%。对镰刀菌(93.96%)、灵芝(100%)、毛毛菌InaCC F228的抑制率(76.64%)均高于条纹法。抗菌试验结果表明，菌株对镰刀菌、灵芝菌和毛毛菌InaCC F228的生长抑制率分别为10.85%、73.31%和30.32%。

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Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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