Genetic characterization of new varieties and hybrids of citrus table fruit through isoenzymes

IF 1.3 4区农林科学 Q3 AGRONOMY

Crop Breeding and Applied Biotechnology Pub Date : 2003-03-30 DOI:10.12702/1984-7033.V03N01A11

C. Oliveira, E. B. Radmann

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引用次数: 0

Abstract

The aim of this study was to establish isoenzymatic patterns to genetically characterize the orange varieties ‘Lane Late’, ‘Navalete’, ‘Navelina’ and ‘Salustiana’, mandarin varieties ‘Clemenules’, ‘Marisol’ and ‘Satsuma Okitsu’, and the hybrids ‘Nova’ and ‘Ortanique’. The following isoenzymatic systems were utilized: alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (APS), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase (6-PGDH), glutamate oxaloacetate transaminase (GOT), isocitrate dehydrogenase (IDH), leucine aminopeptidase (LAP), malate dehydrogenase (MDH), peroxidase (PRX), sorbitol dehydrogenase (SDH), superoxidase dismutase (SOD) and shikimate dehydrogenase (SKDH). Proteins were extracted from leaves and the isoenzymatic polymorphisms were analyzed by polyacrylamide gel electrophoresis. The PGM isoenzyme showed the highest differentiation among the genetic materials tested. The banding profiles observed for PGM, SKDH and EST were satisfactory to characterize the mandarin varieties and hybrids studied. However, no polymorphic bands were found in sweet oranges with the 14 isoenzymes tested.

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柑桔鲜果新品种及杂交种的同工酶遗传特性研究

本研究的目的是建立同酶模式，以遗传表征橙子品种“Lane Late”、“Navalete”、“Navelina”和“Salustiana”，柑橘品种“Clemenules”、“Marisol”和“Satsuma Okitsu”，以及杂交品种“Nova”和“Ortanique”。利用了乙醇脱氢酶(ADH)、酯酶(EST)、酸性磷酸酶(APS)、磷酸葡萄糖异构酶(PGI)、磷酸葡萄糖互变酶(PGM)、6-磷酸葡萄糖酸脱氢酶(6-PGDH)、谷氨酸草酰乙酸转氨酶(GOT)、异柠檬酸脱氢酶(IDH)、白氨酸氨基肽酶(LAP)、苹果酸脱氢酶(MDH)、过氧化物酶(PRX)、山梨醇脱氢酶(SDH)、超氧化酶歧化酶(SOD)和草甘酸脱氢酶(SKDH)等同工酶体系。从叶片中提取蛋白质，用聚丙烯酰胺凝胶电泳分析同工酶多态性。PGM同工酶分化程度最高。PGM、SKDH和EST的条带特征对柑橘品种和杂交种的特征比较满意。但在甜橙中未发现多态性条带。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Breeding and Applied Biotechnology AGRONOMY-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.70

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.