F.C.L. Loureiro , A.M.N. Lima , R. Roque , R.M.P. de Figueiredo , E.U.K. Melcher , M. Borre , C. Thirstrup , H. Neff
{"title":"Simplified Immuno-assay for Rapid Label-free Dengue Serotype Diagnosis: Suppression of Non-specific Binding Interference","authors":"F.C.L. Loureiro , A.M.N. Lima , R. Roque , R.M.P. de Figueiredo , E.U.K. Melcher , M. Borre , C. Thirstrup , H. Neff","doi":"10.1016/j.protcy.2017.04.031","DOIUrl":null,"url":null,"abstract":"<div><p>An easy to implement immunoassay for rapid dengue virus (DENV) serotype diagnosis in the early infection stage is reported. The four-layer assay is immobilized onto a thin gold film and relies on a polymer biochip for optical surface plasmon resonance sensing and detection. The protocol comprises Neutravidin-Biotin mediated DENV monoclonal antibody (MAB<sub>D</sub>) attachment as the functionalized sensing element. The MAB<sub>D</sub>-DENV complex formation results in a pronounced thickness change that is optically detected in real time. The protocol is applicable in presence of strong non-specific binding interference. False positive indications for negative serum and blood control samples were not observed. The method can be modified towards detection of other viruses infections.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":"27 ","pages":"Pages 67-69"},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.031","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Procedia Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212017317300324","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/7/13 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
An easy to implement immunoassay for rapid dengue virus (DENV) serotype diagnosis in the early infection stage is reported. The four-layer assay is immobilized onto a thin gold film and relies on a polymer biochip for optical surface plasmon resonance sensing and detection. The protocol comprises Neutravidin-Biotin mediated DENV monoclonal antibody (MABD) attachment as the functionalized sensing element. The MABD-DENV complex formation results in a pronounced thickness change that is optically detected in real time. The protocol is applicable in presence of strong non-specific binding interference. False positive indications for negative serum and blood control samples were not observed. The method can be modified towards detection of other viruses infections.
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