Coherent Anti-Stokes Raman Scattering Cell Imaging and Segmentation with Unsupervised Data Analysis

Abstract

Offering the high spectral resolution of conventional Raman spectroscopy combined with reduced acquisition time, multiplex coherent anti-Stokes Raman scattering (MCARS) microspectroscopy using sub-nanosecond laser pulses has been accepted as a mature and straightforward technology for label-free bioimaging. In a recently published paper, Philippe Leproux and associates have introduced the combination of the MCARS imaging technique with unsupervised data analysis based on multivariate curve resolution (MCR). Leproux spoke to Spectroscopy about combining the techniques, as well as discussing the potential of the results.