Selective cytotoxic effect of Annona muricataL. in HCC1954 (HER2+) breast cancer cells

IF 0.7 4区医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas Pub Date : 2023-09-30 DOI:10.37360/blacpma.23.22.5.50

Maria del Rosario Huizar Lopez, Josefina Casas Solis, Itzel Citlalli Alcazar Rios, Lucia Barrientos Ramirez, A. Santerre

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Abstract

Annona muricataLinn. (Annonaceae) is a tropical plant with multiple beneficial health effects including anticancer properties. In breast cancer patients, overexpression of the HER2 oncoprotein corresponds to a poor prognosis, thus the main purpose of this study was to evaluate the cytotoxicity of ethanolic extracts from dried and fresh leaf of A. muricataon HER2+ breast cancer cells. MTT assays were performed and IC50determined in HCC1954 (HER2+) cells, as well as in MCF7 (HER-) and peripheral blood mononuclear cells (PBMC) used as controls. Total polyphenol content evaluation and phytochemical screening were also performed. The cytotoxic effect of A. muricataextracts (125-1000 μg/mL) was dose-dependent and cell-type specific. The extracts exhibited higher cytotoxicity against HCC1954 than MCF7 cells, but weak toxicity against PBMC. This is the first report of the cytotoxic effect of A. muricataon HCC1954 cells, highlighting its potential for treating anti-estrogen-resistant breast cancers and low toxicity against PBMC.

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番荔枝的选择性细胞毒作用。在HCC1954 (HER2+)乳腺癌细胞中

番荔枝属muricataLinn。(番荔枝科)是一种热带植物，具有多种有益健康的作用，包括抗癌特性。在乳腺癌患者中，HER2癌蛋白的过表达预示着预后不良，因此本研究的主要目的是评估木犀草干叶和鲜叶乙醇提取物对HER2阳性乳腺癌细胞的细胞毒性。以HCC1954 (HER2+)细胞、MCF7 (HER-)和外周血单个核细胞(PBMC)作为对照，进行MTT试验并测定ic50。还进行了总多酚含量评价和植物化学筛选。木楝提取物(125 ~ 1000 μg/mL)的细胞毒作用具有剂量依赖性和细胞类型特异性。提取物对HCC1954细胞的毒性高于MCF7细胞，对PBMC细胞的毒性较弱。这是第一篇关于A. muricataon对HCC1954细胞的细胞毒性作用的报道，强调了其治疗抗雌激素耐药乳腺癌的潜力和对PBMC的低毒性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas PHARMACOLOGY & PHARMACY-

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas (BLACPMA), [Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants]; currently edited by the publishing house MS-Editions, is a bi-monthly international publication that publishes original peerreviewed research in the field of medicinal and aromatic plants, with nearly 20 years of experience. BLACPMA is a scientific journal that publishes two types of articles: Reviews (only in English) and Original Articles (Spanish or English), its main lines of action being agronomy, anthropology and ethnobotany, industrial applications, botany, quality and standardization, ecology and biodiversity, pharmacology, phytochemistry, pharmacognosy, regulatory and legislative aspects. While all areas of medicinal plants are welcome and the experimental approaches used can be broad and interdisciplinary; other areas of research that are not mentioned depend on the Editorial Committee for their acceptance.