Modulation effects of acid sphingomyelinase/ceramide on the common carotid arteries of simulated weightless rats

中华航空航天医学杂志 Pub Date : 2015-03-15 DOI:10.3760/CMA.J.ISSN.1007-6239.2015.01.001

Yaoping Cheng, Xiao-ping Xie, L. Xue, Zhongchao Wang, Huan Liu, Hai-Jun Zhang, Yao-ming Chang

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引用次数: 1

Abstract

Objective To investigate the alteration of acid sphingomyelinase (ASM)/ ceramide (Cer) and its role in vasoconstriction of the common carotid arteries (CA) of simulated weightless rats. Methods Thirty-six male Sprague-Dawley rats that matched for weight were randomly assigned to suspended group (n=18) and control group (n=18). Suspended group was in simulated weightless by tail suspension for 4 wk. Western blot and immunohistochemistry were conducted to examine the content and distribution of ASM and Cer. Isometric force recording system was used to detect the vasoconstriction and vasodilation functions. Dihydroethidium fluorescent probe was used to evaluate the level of superoxide anion () in arteries. Results Four-week suspension made the ASM protein and Cer level in the CA of suspended group significantly decreased as compared with that of control group (t=3.626-9.922, P<0.01 or <0.05), while vasoconstriction response to KCl and phenylephrine or vasodilation response to acetylcholine and sodium nitroprusside did not change much in two groups. Incubation with C6-ceramide (C6-Cer) did not significantly change the Cer level, vasoconstriction, vasodilation and level of CA in control group but increased Cer level, vasoconstriction response to KCl and level in suspended group (t=3.015-7.088, P<0.05). The suspension influenced less on vasodilation response to acetylcholine and sodium nitroprusside. Conclusions Simulated weightlessness makes the drop on ASM/Cer level and the level in CA as well as its vasoconstriction adaptation. Key words: Weightlessness simulation; Carotid artery, common; Phospholipases; Ceramides; Oxidative stress; Rats, Sprague-Dawley

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酸性鞘磷脂酶/神经酰胺对模拟失重大鼠颈总动脉的调节作用

目的探讨酸性鞘磷脂酶(ASM)/神经酰胺(Cer)在模拟失重大鼠颈总动脉(CA)血管收缩中的变化及其作用。方法选取体重匹配的雄性sd大鼠36只，随机分为悬浮组(n=18)和对照组(n=18)。悬浮组采用尾悬浮模拟失重4周。Western blot和免疫组化检测ASM和Cer的含量和分布。采用等距力记录系统检测血管收缩和血管舒张功能。采用双氢乙啶荧光探针检测大鼠动脉超氧阴离子()水平。结果悬浮4周后，与对照组相比，悬浮组CA中ASM蛋白和Cer水平显著降低(t=3.626 ~ 9.922, P<0.01或<0.05)，而两组对KCl、苯肾上腺素的血管收缩反应和对乙酰胆碱、硝普钠的血管舒张反应无明显变化。c6 -神经酰胺(C6-Cer)对对照组小鼠Cer水平、血管收缩、血管舒张及CA水平无显著影响，而悬浮组小鼠Cer水平、KCl血管收缩反应及CA水平显著升高(t=3.015 ~ 7.088, P<0.05)。该悬浮液对乙酰胆碱和硝普钠的血管舒张反应影响较小。结论模拟失重使大鼠ASM/Cer水平、CA水平及血管收缩适应性下降。关键词:失重模拟;颈动脉，普通;磷脂酶;天然保湿因子;氧化应激;老鼠,Sprague-Dawley

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来源期刊

中华航空航天医学杂志航空航天医学

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2962

期刊介绍： The aim of Chinese Journal of Aerospace Medicine is to combine theory and practice, improve and popularize, actively advocate a hundred flowers bloom and a hundred schools of thought contend, advocate seeking truth from facts, promote the development of the related disciplines of aerospace medicine and human efficiency, and promote the exchange and penetration of aerospace medicine and human efficiency with other biomedical and engineering specialties. Topics of interest for Chinese Journal of Aerospace Medicine include: -The content of the journal belongs to the discipline of special medicine and military medicine, with the characteristics of multidisciplinary synthesis and cross-penetration, and mainly reflected in the aerospace industry, aerospace flight safety and efficiency, as well as the synthesis of special medicine, preventive medicine, environmental medicine, psychology, etc. -Military aeromedicine (Air Force, Navy and Army aeromedicine) and civil aeromedicine, with a balance of aerospace medicine are the strengths of the journal. -The change in aerospace medicine from a focus on promoting physiological compensatory adaptations to enhancing human performance under extreme environmental conditions is what the journal is helping to promote. -The expansion of manuscripts in high altitude medicine is also a special emphasis of the journal.