Prospection of ISSR primers and population genetic characterization of Paratecoma peroba (Record) Kuhlm.

Abstract

The objective of this study is to prospect ISSR primers (Inter Simple Sequence Repeats) and, further genetic characterization in Paratecoma peroba. For this, leaf samples of 20 individuals were collected in a forest fragment, located in a private area, close to the Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo, in the city of Alegre, ES, Brazil. For this, 43 ISSR primers were tested, and 10 primers were selected, which provided a total of 91 bands, with 57 polymorphic bands, resulting in 62.63% polymorphism. The polymorphic information content (PIC = 0.27) indicated moderate informativeness of the primers and, therefore, they are efficient in studies with the species. However, the values found for genetic parameters such as the number of observed (AO = 1.68) and effective (AE = 1.41) alleles and, the genetic diversity indices of Nei (H* = 0.23) and Shannon (I* = 0.35) indicate the occurrence of homozygous loci and low genetic diversity in the population. On the other hand, the genetic structure evaluated by the Bayesian approach revealed the formation of three genetic groups distributed in all sampled individuals, inferring once again about the occurrence of loci in homozygosity. Therefore, the connection of neighbouring fragments and the establishment of individuals obtained from other sources could increase the genetic diversity of the population and reduce the possible effects of depression by inbreeding and genetic drift.