Morphological description based on DUS characters and molecular characterization of ‘Sikkim Primitive’ maize: an endangered unique genetic resource

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-05-10 DOI:10.1017/s1479262122000065

C. Kapoor, Shweta Singh, R. Avasthe, C. Raj, Matber Singh, Hissay L. Lepcha

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引用次数: 1

Abstract

‘Sikkim Primitive’ (SP) maize locally known as ‘murali makkai’ in Sikkim is a unique genetic resource exhibiting prolificacy and excellent popping capacity. Status of SP has reached extinction level due to its very small population size and neglected conservation efforts in-situ. In an initial effort to conserve and revive this landrace, characterization and documentation was carried out with 31 morphologically assayed traits recorded at different growth stages along with molecular characterization with simple-sequence repeat (SSR) markers. Plants exhibited prolificacy (5–6 cobs/plant) and excellent popping capacity along with other distinct traits. Plants were tall with thin stem, loose drooping tassel with anthocyanin coloration present at the base of glumes and in brace roots. Cobs were medium sized carrying small seeds with low test weight (87.90 g). A total of 22 SSR markers show amplification in murali makkai with markers bnlg1083, umc1353, umc1128, bnlg1017, bnlg2077, umc2298 and umc2373 amplified unique amplicons ranging from 100 to 800 bp. The characterized set of traits and molecular characterization for murali makkai will facilitate in utilization for genetic improvement and maintenance of genetic purity.

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濒危独特遗传资源“锡金原始”玉米DUS特征形态描述及分子特征

“锡金原始玉米”(SP)在锡金被称为“murali makkai”，是一种独特的遗传资源，具有高产和优异的拔芽能力。由于其种群规模小，且在当地的保护工作被忽视，SP已达到灭绝的程度。为了保护和恢复这一地方品种，研究人员在不同生长阶段记录了31个形态分析性状，并利用SSR标记进行了分子鉴定。植株表现出多产性(5-6穗子/株)和优异的开穗能力及其他显著性状。植株高，茎细，松散下垂的穗状花序，花青素在颖片基部和支状根中存在。结果显示，22个SSR标记扩增出了100 ~ 800 bp的特异扩增序列，其中，bnlg1083、umc1353、umc1128、bnlg1017、bnlg2077、umc2298和umc2373标记扩增出的特异扩增序列范围为100 ~ 800 bp。对木螺的一组特征和分子特征的研究，将有利于木螺的遗传改良和遗传纯度的维持。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.