Healing effect of Terminalia chebula Retz extract on second-degree burns in rats

IF 0.6 4区医学 Q4 PHARMACOLOGY & PHARMACY

Tropical Journal of Pharmaceutical Research Pub Date : 2016-05-10 DOI:10.4314/TJPR.V15I4.17

P. Meng, Shouguo Ma, Shuwen Huang

{"title":"Healing effect of Terminalia chebula Retz extract on second-degree burns in rats","authors":"P. Meng, Shouguo Ma, Shuwen Huang","doi":"10.4314/TJPR.V15I4.17","DOIUrl":null,"url":null,"abstract":"Purpose : To investigate the healing effect of Terminalia chebula Retz. Extract (TCRE) on seconddegree burns in rats. Methods : Male Sprague Dawley (SD) rats, weighing 200 – 220 g, were subjected to deep seconddegree skin burns by electrical scald instrument. The animals were divided into three groups as follows: (1) second-degree burns model (control) group, (2) burns model treated with 1 % silver sulfadiazine (SSD) group, and (3) burns model treated with 100 mg·mL -1 TCRE group. On days 3, 7 and 14 following the administration of the drug/extract, the wound area and histopathological changes in rat epidermis were evaluated for the various groups. The minimum inhibitory concentration (MIC) of TCRE on Staphyloccocus aureus, Pseudomonas aeruginosa and Escherichia coli were also assessed separately. Results : On day 14, the mean wound area of TCRE treatment group (0.25 ± 0.06 cm 2 ) was significantly smaller than that of the control rats (2.71 ± 0.20 cm 2 , p < 0.01). The histological results indicate that the inflammatory cells disappeared and were replaced by new granulation tissue in the group treated with 100 mg·mL -1 TCRE by day 14. Compared with SSD group rats, the inflammatory cells and fibroblast and granulation tissues of burnt rats treated with 100 mg·mL -1 TCRE were same as those of rats that had no burns. The antibacterial results revealed that the MIC of TCRE on Staphyloccocus aureus, Pseudomonas aeruginosa and Escherichia coli was 3.13, 12.5 and 6.25 mg·mL -1 , respectively. Conclusion : Terminalia chebula Retz. has potentials to be developed as an effective medicinal herb for the treatment of second-degree burns. Keywords : Terminalia chebula, Second-degree burns, Burn wound, Healing, Granulation tissues, Fibroblast, Antibacterial, Inflammatory cells","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2016-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Journal of Pharmaceutical Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4314/TJPR.V15I4.17","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}

引用次数: 3

Abstract

Purpose : To investigate the healing effect of Terminalia chebula Retz. Extract (TCRE) on seconddegree burns in rats. Methods : Male Sprague Dawley (SD) rats, weighing 200 – 220 g, were subjected to deep seconddegree skin burns by electrical scald instrument. The animals were divided into three groups as follows: (1) second-degree burns model (control) group, (2) burns model treated with 1 % silver sulfadiazine (SSD) group, and (3) burns model treated with 100 mg·mL -1 TCRE group. On days 3, 7 and 14 following the administration of the drug/extract, the wound area and histopathological changes in rat epidermis were evaluated for the various groups. The minimum inhibitory concentration (MIC) of TCRE on Staphyloccocus aureus, Pseudomonas aeruginosa and Escherichia coli were also assessed separately. Results : On day 14, the mean wound area of TCRE treatment group (0.25 ± 0.06 cm 2 ) was significantly smaller than that of the control rats (2.71 ± 0.20 cm 2 , p < 0.01). The histological results indicate that the inflammatory cells disappeared and were replaced by new granulation tissue in the group treated with 100 mg·mL -1 TCRE by day 14. Compared with SSD group rats, the inflammatory cells and fibroblast and granulation tissues of burnt rats treated with 100 mg·mL -1 TCRE were same as those of rats that had no burns. The antibacterial results revealed that the MIC of TCRE on Staphyloccocus aureus, Pseudomonas aeruginosa and Escherichia coli was 3.13, 12.5 and 6.25 mg·mL -1 , respectively. Conclusion : Terminalia chebula Retz. has potentials to be developed as an effective medicinal herb for the treatment of second-degree burns. Keywords : Terminalia chebula, Second-degree burns, Burn wound, Healing, Granulation tissues, Fibroblast, Antibacterial, Inflammatory cells

查看原文本刊更多论文

枳椇提取物对大鼠二度烧伤的愈合作用

目的:探讨枳实的治疗作用。中药提取物对大鼠二度烧伤的作用。方法:用电烫伤仪对体重200 ~ 220 g的雄性SD大鼠皮肤进行深二度烧伤。将大鼠分为3组:(1)二级烧伤模型(对照组)，(2)1%磺胺嘧啶银(SSD)组，(3)100 mg·mL -1 TCRE组。给药后第3、7、14天，观察各组大鼠表皮创面面积和组织病理学变化。并分别测定了TCRE对金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌的最低抑菌浓度(MIC)。结果:第14天，TCRE治疗组创面平均面积(0.25±0.06 cm 2)显著小于对照组(2.71±0.20 cm 2, p < 0.01)。组织学结果显示，100 mg·mL -1 TCRE组在第14天炎症细胞消失，并被新的肉芽组织所取代。与SSD组大鼠比较，100 mg·mL -1 TCRE处理烧伤大鼠的炎症细胞、成纤维细胞和肉芽组织与未烧伤大鼠相同。抑菌结果显示，TCRE对金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌的MIC分别为3.13、12.5和6.25 mg·mL -1。结论:chebulula Retz。有潜力发展成为治疗二度烧伤的有效草药。关键词:chebula，二度烧伤，烧伤创面，愈合，肉芽组织，成纤维细胞，抗菌，炎症细胞

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Tropical Journal of Pharmaceutical Research 医学-药学

CiteScore

1.00

自引率

33.30%

发文量

490

审稿时长

4-8 weeks

期刊介绍： We seek to encourage pharmaceutical and allied research of tropical and international relevance and to foster multidisciplinary research and collaboration among scientists, the pharmaceutical industry and the healthcare professionals. We publish articles in pharmaceutical sciences and related disciplines (including biotechnology, cell and molecular biology, drug utilization including adverse drug events, medical and other life sciences, and related engineering fields). Although primarily devoted to original research papers, we welcome reviews on current topics of special interest and relevance.