Tuning physio-mechanical properties of graded micropillar polydimethylsiloxane substrates for cellular attachment and guided migration

IF 0.7 4区材料科学 Q4 METALLURGY & METALLURGICAL ENGINEERING

International Journal of Materials Research Pub Date : 2023-08-30 DOI:10.1557/s43578-023-01142-2

M. Shahriar, M. Uddin, E. Mora, Heqi Xu, Zheng Zhang, Changxue Xu

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引用次数: 0

Abstract

The study of cell–substrate interaction and cellular behavior is critical in tissue engineering and microfluidic research. Since the substrate properties affect the cellular response, it is essential to tune the properties of the polymeric substrate to mimic the native microenvironment for cells. Due to its tunable physical and mechanical properties, polydimethylsiloxane (PDMS) is widely used to study cellular mechanics. This study focused on investigating the effects of substrate stiffness and wettability of PDMS micropillar substrates on cellular response. Mixing different base-to-curing agent ratios of PDMS resulted in different stiffness, while the corona discharge increased the surface wettability. By culturing 3T3 fibroblast cells, it was found that cells preferred a stiffer and more hydrophilic substrate (5:1) compared to the softer and less hydrophilic substrate (20:1) for long-term cell adhesion and migration. This study proves that biomaterials with appropriate stiffness should be chosen to study the cell mechanobiology of this cell line.

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调节微柱聚二甲基硅氧烷底物的物理力学性能，用于细胞附着和引导迁移

细胞-底物相互作用和细胞行为的研究在组织工程和微流体研究中至关重要。由于底物的性质会影响细胞的反应，因此必须调整聚合物底物的性质以模拟细胞的原生微环境。聚二甲基硅氧烷(PDMS)具有可调的物理力学性能，被广泛应用于细胞力学研究。本研究主要研究了PDMS微柱基质的刚度和润湿性对细胞反应的影响。不同基料与固化剂配比的PDMS的刚度不同，电晕放电增加了表面润湿性。通过培养3T3成纤维细胞，我们发现细胞在长期黏附和迁移中更倾向于选择更硬、更亲水的底物(5:1)而不是更软、更不亲水的底物(20:1)。本研究证明，应该选择适当刚度的生物材料来研究该细胞系的细胞力学生物学。

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来源期刊

International Journal of Materials Research 工程技术-冶金工程

CiteScore

1.30

自引率

12.50%

发文量

119

审稿时长

6.4 months

期刊介绍： The International Journal of Materials Research (IJMR) publishes original high quality experimental and theoretical papers and reviews on basic and applied research in the field of materials science and engineering, with focus on synthesis, processing, constitution, and properties of all classes of materials. Particular emphasis is placed on microstructural design, phase relations, computational thermodynamics, and kinetics at the nano to macro scale. Contributions may also focus on progress in advanced characterization techniques. All articles are subject to thorough, independent peer review.