Antimicrobial Effect of Acacia Nilotica Extracts on Endodontic Microorganisms

Abstract

Introduction: Acacia nilotica is widely used in traditional medicine as an anti-inflammatory and anti-bacterial agent. Endodontic irrigations exhibit lot of disadvantages with incomplete removal of bacteria. Sodium Hypochlorite (NaOCl) is the prototype irrigation against primary endodontic infections, while 2% chlorhexidine gluconate (CHX) is the best for secondary endodontic infections. Aim: This study aims to examine the antimicrobial effect of Acacia nilotica against clinical isolates of endodontic microorganisms compared to traditional(NaOCl and CHX). Materials and Methods: A total of 12 random clinical samples were collected for bacterial isolation and identification. Clinical isolates were identified as (Streptococcus viridans& staphylococcus aureus). A well identified candida albicans was also used. Acacia nilotica bark was collected, washed, dried and then grounded to fine powder. Specimens were percolated in methanol, filtrated, and concentrated for crude extract. Autoclaved Mueller Hinton media were used for antimicrobial sensitivity test. Microorganisms were inoculated and test material was loaded as original and 50% for 24h. Antibacterial activity was measured by zone of inhibition Results: All tested agents showed an excellent zone of inhibition against all microorganisms tested with their original concentration. Acacia nilotica showed excellent ZOI with more linear effect in both concentrations against S. viridans and S. aureus. Conclusion: Acacia nilotica has shown an excellent zone of inhibition in both original and 50% concentration, therefore, it may be an excellent substitute or adjunct irrigation solution of hebetic origin in endodontics. We recommend taking this material to the next level as an endodontic irrigant. Key words : Antimicrobial, Acacia Nilotica, Extracts , Endodontic, Microorganisms