APPLICATION OF BIO-FUNGICIDES AS INHIBITING ANTHRACNOSE TO EXTEND THE SELLING TIME OF CALLINA PAPAYA

E. Darmawati, Florensius Labat Bionille
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Abstract

Anthracnose is a disease that attacks papaya fruit while in the field and continues until the fruit is harvested and distributed to the market. Biofungiside made from citronella and green betel was expected to extend the selling papaya at inhibiting the growth of anthracnose. The study was aimed to determine the appropriate concentration of extracts of citronella leaves (Cymbopogon nardus L.) and green betel leaves (Piper betle L.) in inhibiting the growth of C. gloeosporioides as a cause of anthracnose. The extraction was done by boiling the leaves. The test methods used were in vitro and in vivo tests. In vitro tests were carried out to test the effectiveness of citronella leaf extract with concentrations of 5%, 10%, and 10%, 20% concentrations of green betel leaf extract in inhibiting the growth of C. gloeosporioides. The results of the in vitro test showed a significant difference (á = 0.5%) between treatments with the highest effectiveness value being 10% citronella extract with 47.22% growth inhibition of C. gloeosporioides and an average colony diameter of 3.150 cm after 15 days of incubation. The results of the in vivo test, showed significantly different results (á = 0.5%) between papaya fruit soaked with 10% citronella extract and controls, both in the incidence and spread of the disease. The spread of disease in treated papaya fruit was 13.72% while control papaya fruit reached 68.49% at 6 days of storage. Citronella extract with a concentration of 10% can extend the selling time of Calinna papaya (80% maturity index) 6 days longer than the control.
生物杀菌剂抑制炭疽病的应用延长愈伤莲木瓜的销售时间
炭疽病是一种在田间侵害番木瓜果实的疾病,并一直持续到果实收获并分发到市场。从香茅和绿槟榔中提取的生物菌素有望延长销售木瓜在抑制炭疽病生长方面的作用。本研究旨在确定香茅叶(Cymbopogon nardus L.)和绿槟榔叶(Piper betle L.)提取物的适宜浓度对炭疽病菌gloeosporioides生长的抑制作用。提取的方法是把叶子煮沸。试验方法有体外试验和体内试验。通过体外实验,考察了香茅叶提取物浓度为5%、10%和10%、20%的绿槟榔叶提取物对gloeosporioides生长的抑制作用。体外试验结果表明,不同处理间差异显著( = 0.5%),其中10%香茅提取物对黄孢孢子虫生长抑制率最高,为47.22%,培养15 d后平均菌落直径为3.150 cm。体内试验结果显示,10%香茅提取物浸泡的番木瓜果实与对照在发病和传播方面均有显著差异( = 0.5%)。贮藏6 d时,处理过的木瓜果实的病死率为13.72%,而对照木瓜果实的病死率为68.49%。浓度为10%的香茅提取物可使山雀木瓜(80%成熟度指数)的销售时间比对照延长6 d。
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