Application of enterosorbent on the basis of montmorillonite in acute diarrhea (experimental study)

Abstract

Aim . To study the antidiarrheal activity of enterosorbent on the basis of montmorillonite on the model of serotonin-induced diarrhea. Materials and Methods . The study was conducted on laboratory mice of both sexes, acute diarrhea was induced by intraperitoneal injection of serotonin hydrochloride at the dose of 0.32 mg/kg, enterosorbent was introduced orally in the form of aqueous suspension in a wide range of doses 30 minutes before introduction of serotonin. Within 4 hours, the time of onset of diarrhea, the number of defecations, the fluid content in fecal masses were taken into account. Morphological examination of the small intestine was carried out. Results . introduction of serotonin led to diarrhea in 100% of animals within 12.8±1.2 minutes, the number of defecations increased 2.5 times as compared to the intact animals and was 19.5±0.5 times in 4 hours, the fecal masses were predominantly semi-liquid and liquid. Use of enterosorbent coded Crim_04 at the maximum dose led to a delay in diarrhea up to 73.6±4.1 minutes, the number of defecations decreased to 9.4±0.1 with a significant solidification of feces. The antidiarrheal effect of enterosorbent was confirmed morphologically. Conclusions . Enterosorbent on the basis of montmorillonite with laboratory code Crim_04 possesses a dose-dependent antidiarrheal effect in modeling of serotonin-induced diarrhea.