DEVELOPMENT OF FLUORESCENCE POLARIZATION IMMUNOASSAY OF PHTHALATES: COMPARISON OF DIFFERENT DERIVATIVES IN OBTAINING ANTIBODIES AND TRACERS

Abstract

Reagents for fluorescence polarization immunoassay (FPIA) of phthalates have been obtained: phthalate-protein conjugates, polyclonal antibodies, tracers - antigens labeled with fluorescein derivatives. By the example of dibutyl phthalate (DBP) with the use of a portable detector Sentry-200, the FPIA method was developed, which makes it possible to determine DBP in water samples in concentrations 1 μg / ml in 5 min.