Effect of Lycopene on Pharmacokinetic and Pharmacodynamics of Gliclazide in Diabetic Animal Models

Abstract

Background: Diabetes mellitus is one of the metabolic disorders associated with high blood sugar levels. Utilization of natural drugs among patients under diabetes mellitus pharmacotherapy is across the board. Objective: To examine the pk-pd(pharmacodynamic-pharmacokinetic) interactions of lycopene and gliclazide in animal models and to understand the safety & effectiveness. Methods: Single and multiple dose interaction studies were carried out in normal rats, diabetes induced rats and rabbits to evaluate the effect of lycopene on the gliclazide activity. Blood samples from the study animals were used for the estimation insulin and glucose levels by using radioimmunoassay method and chemistry analyzer (automated) respectively. Homeostasis model assessment used for determination of β-cell function. Additionally, sophisticated HP-LC technique used for analysis of diabetic rabbits serum samples for gliclazide. Results: Gliclazide produces significant reduction in blood glucose levels in diabetic animals. However, tests examined from gliclazide in blend with lycopene indicated more prominent diminishment in blood glucose concentration in animals with diabetes. Conclusion: The study concludes that the lycopene along with gliclazide shows the significant pharmacodynamics interaction, but doesn’t establish pharmacokinetics interaction up on single and multiple-dose treatment in animals.