Polymorphic information content of SSR markers for Coffea spp.

IF 1.3 4区农林科学 Q3 AGRONOMY

Crop Breeding and Applied Biotechnology Pub Date : 2010-03-30 DOI:10.12702/1984-7033.V10N01A12

R. F. Missio, E. T. Caixeta, E. M. Zambolim, L. Zambolim, C. Cruz, N. S. Sakiyama

引用次数: 27

Abstract

Thirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzed in 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to 13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values were found for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphic SSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and the leaf rust resistant arabica cultivars.

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咖啡属SSR标记的多态性信息含量。

对24份咖啡树材料中含有(GT)15和(AGG)10重复序列的33条咖啡SSR引物进行了分析。22条引物在种质间呈多态性;等位基因数量在2 ~ 13个之间，平均每个引物有5.1个等位基因。PIC值为0.08 ~ 0.79。平均PIC值最高的品种为canephora(0.46)，最低的品种为arabica(0.22)和triploids(0.22)。本研究利用的多态SSR标记可用于咖啡树的遗传指纹识别，特别是对canephora和抗叶锈病的阿拉比卡品种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Breeding and Applied Biotechnology AGRONOMY-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.70

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.