Dynamics of glutathione- S-transferase gene expression in subacute liver damage caused by acrylamide and on the background of correction

Abstract

The aim of the study was to study the effect of oxymethyluracil complex compounds on the expression of glutathione-S-transferase genes in rat liver under conditions of its toxic damage by acrylamide. Materials and methods. The animals were divided into 5 groups of 6 animals each: control, acrylamide, acrylamide + complex compound of oxymethyluracil with ascorbic acid (MG-1), acrylamide + complex compound of oxymethyluracil with sodium succinate (MG-2), acrylamide + complex compound of oxymethyluracil with acetylcysteine (MG-10). The drugs were administered 1 hour before exposure to the toxicant for 28 days. After the end of the experiment, the animals were decapitated, the liver was removed, which was frozen in liquid nitrogen. Real-time reverse transcription polymerase chain reaction was used to analyze gene expression. Results. Exposure to acrylamide did not significantly affect the expression of the GSTP1, GSTT1, and GSTM1 genes in the liver of rats, however, for all the studied genes, there was a tendency to increase the value of the studied indicator. Prophylactic administration of a complex compound of oxymethyluracil with sodium succinate (MG-2) led to a statistically significant decrease in the transcriptional activity of the GSTM1 gene under conditions of toxic damage to the liver by acrylamide. Conclusion. The results of the study indicate the ability of the MG-2 drug to suppress the expression of the GSTM1 gene in the liver of rats when exposed to acrylamide. Further research is needed to better understand the molecular mechanisms of acrylamide-induced toxicity and to develop new therapeutic approaches to treat liver pathology.