A novel method for Isolating Nanocrystalline Cellulose from Eucalyptus Hardwood

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2019-09-17 DOI:10.4236/jasmi.2019.93006

Jinrong Zhang, Wenbo Zou

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Abstract

The objective of this work is to develop a new method for extracting nanocrystalline cellulose (CNC) from eucalyptus. CNC was isolated from eucalyptus using 6:4 (v/v) tetrahydrofuran/water. The chemical and crystalline structure of the isolated CNC was characterized by zeta potential measurements, Fourier Transform Infrared (FT-IR) and UV-Vis spectroscopies, and X-ray powder diffractometry. The CNC morphology was characterized by Transmission Electron Microscope (TEM) and particle size of CNC was determined by the Dynamic Light Scattering (DLS) method. Cytotoxicity and zebrafish-toxicity experiments were also performed on CNC. The yield of CNC using the new extraction method was about 41%, and the extracted CNC particles were about 20 nm in diameter and about 40 nm long, on average. The zeta potential of the CNC was determined to be -56 eV and the CN particles were uniformly distributed in a suspension of 0.5 wt%. The prepared CNC was a type I polymorph with an intact crystal structure. Toxicity results revealed that 0.5 wt% is a safe CNC concentration, and that 2.5 mg/mL CNC is not teratogenic or lethal to zebrafish embryos or juveniles. The new extraction method developed in this study is environmentally friendly, and easy to operate with relatively low cost.

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从桉树硬木中分离纳米晶纤维素的新方法

本研究的目的是建立一种从桉树中提取纳米晶纤维素的新方法。用6:4 (v/v)四氢呋喃/水从桉树中分离出CNC。通过zeta电位测量、傅里叶变换红外(FT-IR)和紫外-可见光谱以及x射线粉末衍射对分离CNC的化学和晶体结构进行了表征。采用透射电镜(TEM)对CNC的形貌进行了表征，采用动态光散射(DLS)法对CNC的粒径进行了测定。细胞毒性和斑马鱼毒性实验也在CNC上进行。新提取方法的CNC得率约为41%，提取的CNC颗粒直径约20 nm，平均长约40 nm。CNC的zeta电位为-56 eV, CN颗粒均匀分布在0.5% wt%的悬浮液中。制备的CNC为I型晶型，晶体结构完整。毒性结果显示，0.5 wt%是安全的CNC浓度，2.5 mg/mL CNC对斑马鱼胚胎或幼鱼没有致畸或致死作用。本研究开发的新提取方法对环境友好，操作简单，成本相对较低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Analytical Sciences, Methods and Instrumentation

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