Hyperbaric oxygen administration mitigates the expression of kidney injury molecule-1, inflammatory cytokines, and apoptotic markers during cold ischemic time in kidney transplantation

IF 0.7 4区医学 Q4 MARINE & FRESHWATER BIOLOGY

Undersea and Hyperbaric Medicine Pub Date : 2023-01-01 DOI:10.22462/01.01.2023.2

Başak Büyük, Özlem Öztopuz

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引用次数: 0

Abstract

The aim of this study was to investigate the effect of hyperbaric oxygen (HBO2) administration applied during cold ischemic time to organs removed from donors before kidney transplantation. A total of 24 rats were divided into three groups: Group 1 was the control group, Group 2 received 60 minutes of HBO2 at 2.5 atmospheres absolute, and Group 3 received 120 minutes of 2.5 ATA HBO2. The renal artery was entered with a polyethylene catheter and perfused with a standard organ preservation solution. Falcon tubes containing organs obtained from rats in Groups 2 and 3 were placed in a box supported by ice blocks. The temperature was kept constant at 4 °C and the box was placed in a pressure tank with 2.5 ATA HBO2. HBO2 was applied for 60 and 120 minutes, respectively. Organ samples were harvested at the end of 24 hours for histopathological evaluation, immunohistochemical analysis of TNF-α and IL-18, TUNEL analysis for apoptosis, and gene expression levels of kidney injury molecule-1 (KIM-1) and caspase-3. In histopathological examinations, hematoxylin and eosin staining was performed and samples were evaluated for tubular necrosis and vacuolization criteria. Group 2 and Group 3 had significant decreases compared to Group 1 in this regard. Immunohistochemical staining was performed for TNF-α, IL-18, and apoptosis levels; significant decreases were found in Groups 2 and 3. There were significant decreases in Groups 2 and 3 for KIM-1 and caspase-3 gene expression levels compared to Group 1, as well. Thus, it was demonstrated that during the cold ischemic time before kidney transplantation, HBO2 administration to organs removed from donors can reduce apoptotic cell numbers, inflammatory cytokine release, and histopathological damage to the organs as well as decreasing the expression of the KIM-1 gene, which is an indicator of kidney damage.

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高压氧可减轻肾移植冷缺血期间肾损伤分子-1、炎症细胞因子和凋亡标志物的表达

本研究的目的是探讨在冷缺血时间应用高压氧(HBO2)对肾移植前从供体取出的器官的影响。将24只大鼠分为3组:1组为对照组，2组给予2.5个大气压下60分钟的HBO2, 3组给予2.5个大气压下120分钟的HBO2。用聚乙烯导管进入肾动脉，用标准的器官保存液灌注。从第2组和第3组的大鼠身上取下器官，放入装有冰块的盒子中。温度保持在4℃，将箱体置于2.5 ATA HBO2压力罐中。HBO2分别作用60分钟和120分钟。24小时后采集器官标本，进行组织病理学评估、TNF-α和IL-18免疫组化分析、TUNEL细胞凋亡分析、肾损伤分子-1 (KIM-1)和caspase-3基因表达水平分析。在组织病理学检查中，进行苏木精和伊红染色，并评估样本的小管坏死和空泡化标准。与1组相比，2组和3组在这方面有显著降低。免疫组化染色检测TNF-α、IL-18、细胞凋亡水平;第2、3组明显降低。与1组相比，2组和3组的KIM-1和caspase-3基因表达水平也显著降低。由此可见，在肾移植前的冷缺血时期，给药HBO2可以减少供体器官的凋亡细胞数量、炎症细胞因子的释放和对器官的组织病理学损伤，并降低肾损伤指标KIM-1基因的表达。

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来源期刊

Undersea and Hyperbaric Medicine 医学-海洋与淡水生物学

CiteScore

1.60

自引率

11.10%

发文量

审稿时长

>12 weeks

期刊介绍： Undersea and Hyperbaric Medicine Journal accepts manuscripts for publication that are related to the areas of diving research and physiology, hyperbaric medicine and oxygen therapy, submarine medicine, naval medicine and clinical research related to the above topics. To be considered for UHM scientific papers must deal with significant and new research in an area related to biological, physical and clinical phenomena related to the above environments.