Design and fabrication of DNA-based nanostructures using plasmid-protein complex for bio device

Abstract

This report describes the design and fabrication of a DNA nanostructures ("DNA glasses", "DNA serial rings") using plasmid-protein complex. An experiment has been performed to form the DNA ring using two types of 200 bp DNA ("vector DNA") obtained by dissecting pBR322 using restriction enzyme and biotin conjugated 30 bp DNA ("insert DNA"). After ligation and introduction of streptavidin modified gold nanoparticle, the "DNA glasses" and the "DNA serial rings" have been obtained And then, observation of "DNA glasses" using the atomic force microscope (AFM) was carried out.