Fluorescence and surface enhanced Raman scattering dual-model PCR for the detection of DNA barcoding of Asarum heterotropoides

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry Pub Date : 2023-09-01 DOI:10.1016/j.cjac.2023.100304

Chunhao WEI, Aihong WU, Lingling GUO, Liqiang LIU, Chuanlai XU, Hua KUANG, Xinxin XU

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Abstract

Asarum plants containing many functional substances are widely used in traditional prescriptions and tonics. DNA barcoding is a relatively short specific gene segment in the genome which can be used for species identification purposes. The present work selected RNA transcriber type II intron shear enzyme gene (matK) as the target and developed a novel fluorescence and surface enhanced Raman scattering (SERS) dual-model PCR for the detection of Asarum heterotropoides (AH) in plants. The dual-model method achieved specific AH DNA detection with a limit of detection (LOD) of 10⁻³ ng/µL for the fluorescence signal and 10⁻⁴ ng/µL for the SERS signal in the range of 10⁻⁴−10 ng/µL. Agarose gel electrophoresis results and qPCR results showed the novel method was highly specific for AH detection and only the AH was successfully amplified.

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荧光和表面增强拉曼散射双模式PCR检测异角细辛DNA条形码

细辛植物中含有多种功能性物质，是传统方剂和补药中广泛使用的成分。DNA条形码是基因组中相对较短的特定基因片段，可用于物种鉴定。本文以RNA转录子II型内含子剪切酶基因(matK)为靶点，建立了一种荧光和表面增强拉曼散射(SERS)双模式PCR检测植物细辛(Asarum heterotropoides, AH)的方法。双模型方法实现了AH DNA的特异性检测，荧光信号的检测限(LOD)为10−3 ng/µL, SERS信号的检测限(LOD)为10−4 ng/µL。琼脂糖凝胶电泳和qPCR结果表明，该方法对AH检测具有高度特异性，仅成功扩增出AH。

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来源期刊

Chinese Journal of Analytical Chemistry 化学-分析化学

CiteScore

3.60

自引率

25.00%

发文量

17223

审稿时长

35 days

期刊介绍： Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.