Phytochemical Screening, and In Vitro Evaluation of Antioxidant Activity of Lolium Perenne

Journal of advanced Biomedical and Pharmaceutical Sciences Pub Date : 2023-06-14 DOI:10.21608/jabps.2023.198473.1185

Mahmoud Nagaty Mahrous, E. Attia, Mohamed S. A. Abdelkader, Ashraf Hamed

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引用次数: 1

Abstract

The current study aimed to investigate the preliminary phytochemical screening, quantify the total phenolic (TPC), flavonoidal (TFC) contents and evaluate the antioxidant activities of the total methanol extract (ME) of the aerial parts and its derived fractions of Lolium perenne L. ( L. perenne ). The preliminary phytochemical screening of ME displayed the presence of carbohydrates and/or glycosides, steroids and/or triterpenes, phenolic compounds, proteins and/or amino acids, flavonoids, and quinones. Dichloromethane (DFr) and ethyl acetate (EFr) fractions showed the highest TPC (82.42±2.70 and 70.85±2.27 mg gallic acid equivalent (GAE)/g dried sample), respectively. Also, DFr and EFr displayed the highest TFC (115.65±6.43 and 146.00±1.06 mg rutin equivalent (RE)/g dried sample), respectively. All samples showed variable antioxidant properties. While EFr, followed by DFr, demonstrated the least IC 50 value of 120.04±0.43 and 166.81±0.53 µg/mL, respectively, indicating their high antioxidant potential.

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黑麦草的植物化学筛选及体外抗氧化活性评价

本研究旨在对黑麦草(Lolium perenne L.)地上部及其衍生部位的总酚(TPC)、总黄酮(TFC)含量进行初步的植物化学筛选，并对其总甲醇提取物(ME)的抗氧化活性进行评价。对ME的初步植物化学筛选显示存在碳水化合物和/或糖苷、类固醇和/或三萜、酚类化合物、蛋白质和/或氨基酸、类黄酮和醌。二氯甲烷(DFr)和乙酸乙酯(EFr)组分的TPC最高，分别为82.42±2.70和70.85±2.27 mg没食子酸当量(GAE)/g干燥样品。DFr和EFr的TFC含量最高，分别为115.65±6.43和146.00±1.06 mg芦丁当量(RE)/g。所有样品均表现出不同的抗氧化性能。EFr和DFr的IC 50值最小，分别为120.04±0.43µg/mL和166.81±0.53µg/mL，表明其具有较高的抗氧化能力。

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Journal of advanced Biomedical and Pharmaceutical Sciences

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