Real-time PCR High-resolution Melting Analysis for the Species Identification of Meat Products: Focusing on Food Safety and Detection of Meat Adulterations

Thrita Pub Date : 2021-06-20 DOI:10.5812/thrita.112550
Peyman Gholamnezhad, H. Ahari, G. N. Brujeni, S. Anvar, A. Motallebi
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引用次数: 3

Abstract

Background: Real-time polymerase chain reaction (PCR) and high-resolution melting (HRM) analysis are currently considered as reliable techniques for the species identification of meat-based products and widely used to detect meat adulteration. Objectives: To examine the validity of real-time PCR and HRM analysis to identify meat species in meat-based products. Methods: Meat samples from five species (i.e., cattle, sheep, chicken, turkey, and wild pig) were purchased. Minced meat from the animal species of interest was prepared at the purities of 10%, and 20% and also were prepared as single and mixtures of two species. For molecular assessments, DNA samples were extracted from all the meat samples and subjected to real-time PCR by amplifying a mitochondrial cytochrome b specific for each species. Results: All the meat species studied in this research were successfully detected in the mixed meat samples when separately examined by real-time PCR. High-resolution melting analysis showed that all the meat species of interest were efficiently distinguished when examined simultaneously. Conclusions: The data presented here shows that the real-time PCR and HRM analysis are reliable methods for the identification of meat species used in meat products.
实时荧光定量PCR高分辨率熔解分析用于肉制品种类鉴定:着眼于食品安全和肉类掺假检测
背景:实时聚合酶链反应(PCR)和高分辨率熔融(HRM)分析目前被认为是肉类产品物种鉴定的可靠技术,并广泛用于肉类掺假检测。目的:检验实时荧光定量PCR和人力资源管理分析在肉类产品中鉴定肉类种类的有效性。方法:采集牛、羊、鸡、火鸡肉、野猪5种肉类样品。以10%和20%的纯度制备了所研究动物物种的肉糜,并将其制备为单一和两种动物物种的混合物。为了进行分子评估,从所有肉类样品中提取DNA样本,并通过扩增每个物种特异性的线粒体细胞色素b进行实时PCR。结果:通过实时荧光定量PCR分别检测,本研究所研究的肉品种在混合肉品中均能检出。高分辨率熔化分析表明,当同时检测时,所有感兴趣的肉类种类都能有效区分。结论:本文的数据表明,real-time PCR和HRM分析是肉制品中肉类品种鉴定的可靠方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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