Rice blast resistance gene profiling of Thai, Japanese and International rice varieties using gene-specific markers

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-05-12 DOI:10.1017/s1479262122000089

Wattanaporn Teerasan, Pattaraborn Moonsap, Apinya Longya, K. Damchuay, S. Ito, P. Tasanasuwan, Sureeporn Kate-ngam, C. Jantasuriyarat

{"title":"Rice blast resistance gene profiling of Thai, Japanese and International rice varieties using gene-specific markers","authors":"Wattanaporn Teerasan, Pattaraborn Moonsap, Apinya Longya, K. Damchuay, S. Ito, P. Tasanasuwan, Sureeporn Kate-ngam, C. Jantasuriyarat","doi":"10.1017/s1479262122000089","DOIUrl":null,"url":null,"abstract":"\n Rice blast disease, caused by Magnaporthe oryzae, is one of the most damaging diseases of rice worldwide. Cultivation of rice varieties carrying resistance genes is the most economic and successful strategy to control the disease. In this study, 451 rice varieties from around the world including 363 Thai landrace rice varieties, 21 Thai improved rice varieties, 43 Japanese rice varieties and 24 worldwide rice varieties were screened by PCR technique using gene-specific markers for 10 rice blast resistance genes: Pi9, Piz-t, Pi50, Pigm(t), Pid2, Pid3, Pia, Pik, Pi54 and Pita. The results showed that 382 (99.48%) Thai rice varieties have at least one resistance gene and two rice varieties, ‘Hom’ and ‘Bak muay’, contained eight out of ten screened rice blast resistance genes. 320 rice varieties (83.33%) contained three or more rice blast resistance genes. The frequency of the rice blast resistance gene ranges from 87.76–9.64 per cent, of which the Pid3 gene has the highest frequency and the Pi54 gene has the lowest frequency. Two major resistance genes, found in Japanese rice varieties, are the Pik gene (76.74%) and the Pi9 gene (72.09%). While two major resistance genes, found in the international rice varieties are the Pi9 gene (66.67%) and the Pi54 gene (62.50%). The disease resistance gene profile of each rice variety obtained from this study will benefit the rice blast resistant breeding programme in the future.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2022-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1017/s1479262122000089","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 2

Abstract

Rice blast disease, caused by Magnaporthe oryzae, is one of the most damaging diseases of rice worldwide. Cultivation of rice varieties carrying resistance genes is the most economic and successful strategy to control the disease. In this study, 451 rice varieties from around the world including 363 Thai landrace rice varieties, 21 Thai improved rice varieties, 43 Japanese rice varieties and 24 worldwide rice varieties were screened by PCR technique using gene-specific markers for 10 rice blast resistance genes: Pi9, Piz-t, Pi50, Pigm(t), Pid2, Pid3, Pia, Pik, Pi54 and Pita. The results showed that 382 (99.48%) Thai rice varieties have at least one resistance gene and two rice varieties, ‘Hom’ and ‘Bak muay’, contained eight out of ten screened rice blast resistance genes. 320 rice varieties (83.33%) contained three or more rice blast resistance genes. The frequency of the rice blast resistance gene ranges from 87.76–9.64 per cent, of which the Pid3 gene has the highest frequency and the Pi54 gene has the lowest frequency. Two major resistance genes, found in Japanese rice varieties, are the Pik gene (76.74%) and the Pi9 gene (72.09%). While two major resistance genes, found in the international rice varieties are the Pi9 gene (66.67%) and the Pi54 gene (62.50%). The disease resistance gene profile of each rice variety obtained from this study will benefit the rice blast resistant breeding programme in the future.

查看原文本刊更多论文

利用基因特异性标记分析泰国、日本和国际水稻品种抗稻瘟病基因

稻瘟病是由稻瘟病菌(Magnaporthe oryzae)引起的稻瘟病，是世界范围内危害最大的水稻病害之一。培育携带抗性基因的水稻品种是防治稻瘟病最经济、最成功的策略。本研究利用PCR技术对来自世界各地的451个水稻品种(包括363个泰国地方水稻品种、21个泰国改良水稻品种、43个日本水稻品种和24个世界水稻品种)的10个稻瘟病抗性基因(Pi9、pizt、Pi50、Pigm(t)、Pid2、Pid3、Pia、Pik、Pi54和Pita)进行了基因特异性标记筛选。结果表明，382个(99.48%)泰国水稻品种至少含有1个稻瘟病抗性基因，其中‘洪’和‘白梅’两个品种含有8个稻瘟病抗性基因。320个水稻品种(83.33%)含有3个或3个以上抗稻瘟病基因。稻瘟病抗性基因出现频率在87.76% ~ 9.64%之间，其中Pid3基因出现频率最高，Pi54基因出现频率最低。在日本水稻品种中发现的两个主要抗性基因是Pik基因(76.74%)和Pi9基因(72.09%)。在国际水稻品种中发现的两个主要抗性基因是Pi9基因(66.67%)和Pi54基因(62.50%)。本研究获得的每个水稻品种的抗病基因谱将有利于今后水稻抗稻瘟病育种计划的开展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.