L-Ascorbic acid modulates the cytotoxic and genotoxic effects of salinity in barley meristem cells by regulating mitotic activity and chromosomal aberrations

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2023-02-12 DOI:10.36253/caryologia-1791

S. Tabur, Naime Büyükkaya Bayraktar, Serkan Özmen

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引用次数: 0

Abstract

The objective of the present study was to with all details explain of the efficiency of L-ascorbic acid (L-AsA) also known as vitamin C on cytotoxicity and genotoxicity induced by salt stress in the barley apical meristems. As a result of the statistical analysis salt stress caused a significant (P ≤ 0.05) decrease in mitotic index of barley seeds depending on concentration increase, while the frequency of chromosomal aberration (CA) increased. In addition, it was determined that mitotic index value was decreased by 46% with 1 μM L-AsA supplementation as compared to control and chromosomal abnormalities were increased by 8.96% as well as. However, in the case of simultaneously application of 1 μM L-AsA and different salt concentrations, the high salt concentrations exhibited an excellent success according to low salt concentrations in alleviating the mitodepressive effect of salt stress. Moreover, the frequency of chromosomal aberrations in the root meristem cells of those seeds with 1 μM L-AsA supplementation germinated at different salt concentrations was substantially reduced compared to own control group (alone 1 μM L-AsA pretreatment). The 1 μM L-AsA pretreatment at the highest salt concentration (at 0.40 M) was showed an excellent success by reducing the frequency of the chromosomal aberrations by approximately 90 %. Different salt concentrations and/or 1 μM L-AsA supplementation caused micronuclei and granulation as well as various chromosomal aberrations in prophase, metaphase, anaphase and telophase.

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l -抗坏血酸通过调节有丝分裂活性和染色体畸变来调节盐度对大麦分生组织细胞的细胞毒性和基因毒性作用

本研究的目的是详细解释l -抗坏血酸(L-AsA)(又称维生素C)对盐胁迫下大麦根尖分生组织细胞毒性和遗传毒性的影响。统计分析表明，盐胁迫导致大麦种子有丝分裂指数随盐胁迫浓度的升高而显著降低(P≤0.05)，染色体畸变(CA)频率升高。此外，与对照组相比，添加1 μ L-AsA后，有丝分裂指数值降低了46%，染色体异常增加了8.96%。然而，在同时施用1 μ L-AsA和不同盐浓度的情况下，相对于低盐浓度，高盐浓度在缓解盐胁迫的线粒体抑制效应方面表现出优异的效果。此外，与单独处理1 μ L-AsA的对照组相比，添加1 μ L-AsA的种子在不同盐浓度下发芽的根分生组织细胞中染色体畸变的频率显著降低。在最高盐浓度(0.40 M)下进行1 μM L-AsA预处理，可将染色体畸变的频率降低约90%。不同的盐浓度和/或添加1 μM L-AsA可引起前、中期、后期和末期的微核和肉芽形成以及各种染色体畸变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.